University of Granada, Faculty of Dentistry, Colegio Máximo de Cartuja s/n, Granada 18071, Spain.
University of Granada, Faculty of Dentistry, Colegio Máximo de Cartuja s/n, Granada 18071, Spain; Medicina Clínica y Salud Pública PhD Programme, University of Granada, Granada 18071, Spain.
J Dent. 2024 Nov;150:105334. doi: 10.1016/j.jdent.2024.105334. Epub 2024 Aug 30.
To investigate the effect of dentin infiltration with polymeric nanoparticles (NPs) doped with tideglusib (TDg) (TDg-NPs) on hydroxyapatite formation, crystallinity and elasticity of conditioned resin-dentin interfaces.
Dentin conditioned surfaces were infiltrated with NPs or TDg-NPs. Bonded interfaces were created, stored for 24 h and submitted to mechanical and thermal challenging. Resin-dentin interfaces were evaluated through nanoindentation to determine the modulus of elasticity, X-ray diffraction and transmission electron microscopy through selected area diffraction and bright-filed imaging.
TDg-NPs provoked peaks narrowing after the diffraction-intensity analysis that corresponded with high crystallinity, with an increased modulus of Young after load cycling in comparison with the samples treated with undoped NPs. New minerals, in the group of TDg-NPs, showed the greatest both deviation of line profile from perfect crystal diffraction and dimension of the lattice strain, i.e., crystallite, grain size and microstrain and 002 plane-texture. The new minerals generated after TDg-NPs application and mechanical loading followed a well defined lineation. Undoped NPs mostly produced small hydroxyapatite crystallites, non crystalline or amorphous in nature with poor maturity.
Tideglusib promoted the precipitation of hydroxyapatite, as a major crystalline phase, at the intrafibrillar compartment of the collagen fibrils, enabling functional mineralization. TDg-NPs facilitated nucleation of crystals randomly oriented, showing less structural variation in angles and distances that improved crystallographic relative order of atoms and maturity. Nanocrystals inducted by TDg-NPs were hexagonal prisms of submicron size. Thermal challenging of dentin treated with TDg-NPs have provoked a decrease of functional mineralization and crystallinity, associated to immature hydroxyapatite.
New polycrystalline lattice formation generated after TDg-NPs infiltration may become correlated with high mechanical performance. This association can be inferred from the superior crystallinity that was obtained in presence of tideglusib. Immature crystallites formed in dentin treated with undoped NPs will account for a high remineralizing activity.
研究掺杂替度鲁肽（TDg）的聚合物纳米颗粒（NPs）（TDg-NPs）对牙本质中羟磷灰石形成、结晶度和弹性的影响。
用 NPs 或 TDg-NPs 对牙本质表面进行渗透处理。制作粘结界面，保存 24 小时，然后进行机械和热挑战。通过纳米压痕法评估树脂-牙本质界面的弹性模量，通过 X 射线衍射和电子显微镜选区衍射及明场成像分析。
TDg-NPs 在进行衍射强度分析后出现峰变窄，表明其结晶度较高，与用未掺杂 NPs 处理的样品相比，在循环加载后杨氏模量增加。与 TDg-NPs 处理组相比，新生成的矿物表现出更大的线轮廓偏离完美晶体衍射和晶格应变的尺寸，即晶粒尺寸、晶粒度和微应变以及 002 面织构。TDg-NPs 应用和机械加载后生成的新矿物沿着一条明确的线化方向生长。未掺杂 NPs 主要生成小的羟磷灰石晶体，本质上是非晶或非晶态，成熟度较差。
替度鲁肽促进了羟磷灰石作为主要结晶相在胶原纤维原纤维间隔内的沉淀，实现了功能性矿化。TDg-NPs 促进了随机取向晶体的成核，显示出角度和距离的结构变化较小，提高了原子的结晶相对有序性和成熟度。TDg-NPs 诱导的纳米晶体为亚微米尺寸的六方棱柱体。经 TDg-NPs 处理的牙本质经热挑战后，功能矿化和结晶度下降，与不成熟的羟磷灰石有关。
TDg-NPs 渗透后形成的新多晶晶格可能与高机械性能相关。这种关联可以从替度鲁肽存在时获得的更高结晶度推断出来。未掺杂 NPs 处理的牙本质中形成的不成熟晶体将具有较高的再矿化活性。
