Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China.
Sci Rep. 2024 Apr 28;14(1):9735. doi: 10.1038/s41598-024-60520-w.
To investigate the Raman spectral features of orbital rhabdomyosarcoma (ORMS) tissue and normal orbital tissue in vitro, and to explore the feasibility of Raman spectroscopy for the optical diagnosis of ORMS. 23 specimens of ORMS and 27 specimens of normal orbital tissue were obtained from resection surgery and measured in vitro using Raman spectroscopy coupled to a fiber optic probe. The important spectral differences between the tissue categories were exploited for tissue classification with the multivariate statistical techniques of principal component analysis (PCA) and linear discriminant analysis (LDA). Compared to normal tissue, the Raman peak intensities located at 1450 and 1655 cm were significantly lower for ORMS (p < 0.05), while the peak intensities located at 721, 758, 1002, 1088, 1156, 1206, 1340, 1526 cm were significantly higher (p < 0.05). Raman spectra differences between normal tissue and ORMS could be attributed to the changes in the relative amounts of biochemical components, such as nucleic acids, tryptophan, phenylalanine, carotenoid and lipids. The Raman spectroscopy technique together with PCA-LDA modeling provides a diagnostic accuracy of 90.0%, sensitivity of 91.3%, and specificity of 88.9% for ORMS identification. Significant differences in Raman peak intensities exist between normal orbital tissue and ORMS. This work demonstrated for the first time that the Raman spectroscopy associated with PCA-LDA diagnostic algorithms has promising potential for accurate, rapid and noninvasive optical diagnosis of ORMS at the molecular level.
为了研究眼眶横纹肌肉瘤（ORMS）组织和正常眼眶组织的拉曼光谱特征，探索拉曼光谱用于 ORMS 光学诊断的可行性。本研究从手术切除标本中获得 23 例 ORMS 组织和 27 例正常眼眶组织，使用光纤探针耦合拉曼光谱仪进行体外测量。利用主成分分析（PCA）和线性判别分析（LDA）等多元统计技术，挖掘组织分类的重要光谱差异。与正常组织相比，ORMS 的拉曼峰强度位于 1450 和 1655cm 处显著降低（p<0.05），而位于 721、758、1002、1088、1156、1206、1340、1526cm 处的拉曼峰强度显著升高（p<0.05）。正常组织和 ORMS 之间的拉曼光谱差异可归因于核酸、色氨酸、苯丙氨酸、类胡萝卜素和脂质等生化成分相对含量的变化。拉曼光谱技术结合 PCA-LDA 模型可实现 90.0%的诊断准确率、91.3%的灵敏度和 88.9%的特异性，用于 ORMS 识别。正常眼眶组织和 ORMS 之间存在显著的拉曼峰强度差异。本研究首次证明，与 PCA-LDA 诊断算法相结合的拉曼光谱技术具有在分子水平上准确、快速、无创地光学诊断 ORMS 的潜在应用价值。
