Srisuksai Krittika, Limudomporn Paviga, Kovitvadhi Uthaiwan, Thongsuwan Khunakon, Imaram Witcha, Lertchaiyongphanit Ratchaphon, Sareepoch Tharinee, Kovitvadhi Attawit, Fungfuang Wirasak
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Vet World. 2024 Mar;17(3):518-526. doi: 10.14202/vetworld.2024.518-526. Epub 2024 Mar 5.
, a black soldier fly, is widely recognized for sustainable recycling of organic waste. Black soldier fly larvae (BSFLs) can consume various types of biowastes and convert them into nutrient-rich biomass, including proteins, lipids, chitin, and minerals. This study investigated the best extraction method by comparing the fatty acid profiles, percentage yield, and antioxidant properties of BSFL oil extracted using different extraction methods.
The physicochemical properties, fatty acid profile, and free radical scavenging ability of BSFL oil were analyzed using six extraction methods.
Ultrasonic extraction with hexane resulted in the highest yields compared with different extraction methods. Lauric acid (28%-37%) was the most abundant fatty acid in all extracts, followed by palmitic acid, myristic acid, oleic acid, and linoleic acid. Compared with other methods, aqueous extraction showed the highest lauric acid composition and free radical scavenging activities. In addition, high-temperature aqueous extraction resulted in higher oil yield and free radical scavenging activities than low-temperature extraction.
High-temperature aqueous extraction is the best extraction method because it is rich in lauric acid, has antioxidant ability, and can be further developed to produce novel sustainable biomaterials for humans and animals.
黑水虻作为一种可持续回收有机废弃物的昆虫,已得到广泛认可。黑水虻幼虫(BSFL)能够消耗各类生物废弃物,并将其转化为富含蛋白质、脂质、几丁质和矿物质等营养成分的生物质。本研究通过比较不同提取方法所提取的黑水虻幼虫油的脂肪酸组成、得率百分比及抗氧化性能,探究最佳提取方法。
采用六种提取方法对黑水虻幼虫油的理化性质、脂肪酸组成及自由基清除能力进行分析。
与其他提取方法相比,正己烷超声提取法的得率最高。月桂酸(28%-37%)是所有提取物中含量最丰富的脂肪酸,其次是棕榈酸、肉豆蔻酸、油酸和亚油酸。与其他方法相比,水提法的月桂酸含量最高,自由基清除活性也最高。此外,高温水提法比低温水提法的出油率和自由基清除活性更高。
高温水提法是最佳提取方法,因为其富含月桂酸,具有抗氧化能力,可进一步开发用于生产新型可持续的人类和动物生物材料。