Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Nur, Iran.
Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Nur, Iran.
Carbohydr Polym. 2020 Aug 1;241:116358. doi: 10.1016/j.carbpol.2020.116358. Epub 2020 Apr 26.
The polysaccharide isolated from F. gummosa (FGP) was found homogenous with a weight average molecular weight (M) of 50.0 × 10 g/mol and radius of gyration (R) of 105.3 nm. The FGP was an arabinogalactan with a backbone formed of →6)-β-Galp-1→ residues having random branching points at C-3 extended with either β-Galp-(1→3)-β-Galp-(1→ or α-Araf-(1→ side chain residues. FGP exhibited proliferative effect on RAW264.7 cells and induced macrophages to exert proinflammatory response releasing NO and up-regulating the transcription of cytokines including TNF-α, IL-1β, IL-6 and IL-12. The FGP induced NK-92 cells to up-regulate the expressions of TNF-α, IFN-γ, granzyme-B, perforin, NKG2D and FasL. The presence of p-NF- κB, p-ERK, p-JNK and p-p38 in RAW264.7 and NK-92 cells indicated their activation through NF-κB and MAPKs signaling pathways. These findings suggested that polysaccharides from F. gummosa are potent in boosting immune system and thus may be considered for further studies of biomedical applications.
从 F. gummosa 中分离得到的多糖（FGP）具有均一性，重均分子量（M）为 50.0×10 g/mol，回转半径（R）为 105.3nm。FGP 是一种阿拉伯半乳聚糖，其主链由→6）-β-Galp-1→残基组成，在 C-3 处具有随机分支点，延伸的支链分别为β-Galp-（1→3）-β-Galp-（1→或α-Araf-（1→侧链残基。FGP 对 RAW264.7 细胞具有增殖作用，并诱导巨噬细胞发挥促炎反应，释放 NO 并上调细胞因子 TNF-α、IL-1β、IL-6 和 IL-12 的转录。FGP 诱导 NK-92 细胞上调 TNF-α、IFN-γ、颗粒酶-B、穿孔素、NKG2D 和 FasL 的表达。RAW264.7 和 NK-92 细胞中 p-NF-κB、p-ERK、p-JNK 和 p-p38 的存在表明它们通过 NF-κB 和 MAPKs 信号通路被激活。这些发现表明，来自 F. gummosa 的多糖具有增强免疫系统的功效，因此可能值得进一步研究其在生物医学中的应用。
