Edmunds H N, Baden J M, Simmon V F
Anesthesiology. 1979 Nov;51(5):424-9. doi: 10.1097/00000542-197911000-00011.
The mutagenicities of two volatile metabolites of halothane, 2-chloro-1,1,1-trifluoroethane (CF3CH2Cl) and 2-chloro-1,1-difluoro-ethylene (CF2CHCl), and a presumed halothane metabolite, 2-bromo-2-chloro-1,1-difluoroethylene (CF2CBrCl), were investigated in the bacterial assay system developed by Ames and co-workers. Both gas-phase and liquid culture tests were made. In addition, mutagenicity of CF2CBrCl was studied in a modified Ames test system using rapidly growing cells in enriched liquid medium. The purity of tested compounds was verified by combined gas chromatography and mass spectrometry. In the standard Ames test, CF3CH2Cl and CF2CBrCl were not mutagenic, and CF2CHCl was only weakly mutagenic. CF2CBrCl was detectable as weakly mutagenic, however, in the modified Ames test using rapidly growing cells. Although these results are reassuring, the effects of long-term exposure to halogenated anesthetics are still not fully known.
研究了氟烷的两种挥发性代谢产物2-氯-1,1,1-三氟乙烷（CF3CH2Cl）和2-氯-1,1-二氟乙烯（CF2CHCl）以及一种推测的氟烷代谢产物2-溴-2-氯-1,1-二氟乙烯（CF2CBrCl）在由艾姆斯及其同事开发的细菌检测系统中的致突变性。进行了气相和液体培养试验。此外，在改良的艾姆斯试验系统中，使用富集液体培养基中快速生长的细胞研究了CF2CBrCl的致突变性。通过气相色谱和质谱联用验证了受试化合物的纯度。在标准艾姆斯试验中，CF3CH2Cl和CF2CBrCl无致突变性，CF2CHCl仅有微弱的致突变性。然而，在使用快速生长细胞的改良艾姆斯试验中，CF2CBrCl可检测到微弱的致突变性。尽管这些结果令人放心，但长期接触卤化麻醉剂的影响仍不完全清楚。
