Patil Girish, Agarwala Pragya, Das Padma, Pathak Swati
Microbiology, All India Institute of Medical Sciences, Raipur, Raipur, IND.
Cureus. 2024 Mar 30;16(3):e57250. doi: 10.7759/cureus.57250. eCollection 2024 Mar.
Coagulase-negative staphylococci (CoNS) are one of the frequently isolated bacteria from blood cultures. Since they are part of the normal skin flora, they were previously considered contaminants. But now, they can be considered as established pathogens causing bloodstream infection (BSI). This study aims to estimate the prevalence of CoNS in BSI cases.
This study was conducted at the Microbiology Department, All India Institute of Medical Sciences (AIIMS), Raipur, India, for eight months (January 2022 to August 2022). Data were collected retrospectively from medical and laboratory records. Paired blood cultures from 5085 clinically suspected sepsis cases were subjected to aerobic culture for five days in the BacT ALERT 3D system. Pathogenicity was established after recovery of CoNS from paired blood cultures of symptomatic patients.
CoNS were isolated from 2.35% of patients, the most common species being (51.67%). About 90% of isolates were methicillin-resistant. All the isolates were susceptible to linezolid, teicoplanin, and vancomycin, except one isolate of which was intermediate to vancomycin. Minimum inhibitory concentration (MIC) 50 and MIC 90 for vancomycin were 1 ug/ml and 2 ug/ml, respectively. Conclusion: Paired blood cultures are necessary to determine the pathogenicity of CoNS in BSI cases. A high prevalence of methicillin resistance, accompanied by high resistance rates to other non-beta lactam antibiotics, warrants the strict implementation of antimicrobial stewardship practices.
凝固酶阴性葡萄球菌（CoNS）是血培养中经常分离出的细菌之一。由于它们是正常皮肤菌群的一部分，以前被认为是污染物。但现在，它们可被视为引起血流感染（BSI）的确立病原体。本研究旨在评估BSI病例中CoNS的患病率。
本研究在印度赖布尔全印医学科学研究所（AIIMS）微生物学系进行，为期八个月（2022年1月至2022年8月）。数据从医疗和实验室记录中回顾性收集。对5085例临床疑似败血症病例的配对血培养物在BacT ALERT 3D系统中进行为期五天的需氧培养。从有症状患者的配对血培养物中分离出CoNS后确定其致病性。
2.35%的患者分离出CoNS，最常见的菌种是 （51.67%）。约90%的分离株对甲氧西林耐药。除一株 对万古霉素中介外，所有分离株对利奈唑胺、替考拉宁和万古霉素敏感。万古霉素的最低抑菌浓度（MIC）50和MIC 90分别为1微克/毫升和2微克/毫升。结论：配对血培养对于确定BSI病例中CoNS的致病性是必要的。甲氧西林耐药率高，同时对其他非β-内酰胺类抗生素耐药率也高，这就需要严格实施抗菌药物管理措施。
