School of Material Science and Engineering, Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300350, China.
NHC Key Laboratory of Critical Care Medicine, Department of Neurosurgery, Binhai Hospital of Tianjin Medical University General Hospital, Tianjin 300480, China.
Biomater Sci. 2024 Jun 11;12(12):3141-3153. doi: 10.1039/d4bm00164h.
Intestine damage is an acute abdominal disease that usually requires emergency sealing. However, traditional surgical suture not only causes secondary damage to the injured tissue, but also results in adhesion with other tissues in the abdominal cavity. To this end, a thermally reversible injectable gelatin-based hydrogel adhesive (GTPC) is constructed by introducing transglutaminase (TGase) and proanthocyanidins (PCs) into a gelatin system. By reducing the catalytic activity of TGase, the density of covalent and hydrogen bond crosslinking in the hydrogel can be regulated to tune the sol-gel transition temperature of gelatin-based hydrogels above the physiological temperature (42 °C) without introducing any synthetic small molecules. The GTPC hydrogel exhibits good tissue adhesion, antioxidant, and antibacterial properties, which can effectively seal damaged intestinal tissues and regulate the microenvironment of the damaged site, promoting tissue repair and regeneration. Intriguingly, temperature-induced hydrogen bond disruption and reformation confer the hydrogel with asymmetric adhesion properties, preventing tissue adhesion when applied . Animal experiment outcomes reveal that the GTPC hydrogel can seal the damaged intestinal tissue firmly, accelerate tissue healing, and efficiently prevent postoperative adhesion.
肠损伤是一种急性腹部疾病，通常需要紧急密封。然而，传统的手术缝合不仅会对受损组织造成二次损伤，还会导致与腹腔内其他组织粘连。为此，通过向明胶体系中引入转谷氨酰胺酶（TGase）和原花青素（PCs），构建了一种热可逆的可注射明胶基水凝胶粘合剂（GTPC）。通过降低 TGase 的催化活性，可以调节水凝胶中共价和氢键交联的密度，从而将基于明胶的水凝胶的溶胶-凝胶转变温度调节到生理温度（42°C）以上，而无需引入任何合成小分子。GTPC 水凝胶具有良好的组织黏附性、抗氧化和抗菌性能，可有效密封受损的肠组织，并调节损伤部位的微环境，促进组织修复和再生。有趣的是，温度诱导的氢键断裂和形成赋予了水凝胶不对称的黏附性能，在应用时防止组织黏附。动物实验结果表明，GTPC 水凝胶可牢固密封受损的肠组织，加速组织愈合，并有效防止术后粘连。
