Yang Xiaoqian, Niu Yun, Fan Yingrun, Zheng Tingting, Fan Jiangping
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Int J Biol Macromol. 2024 Jun;269(Pt 1):131928. doi: 10.1016/j.ijbiomac.2024.131928. Epub 2024 Apr 28.
To reduce pollution caused by traditional plastic packaging and preparation of silver nanoparticles (AgNPs), this work aims to develop biological macromolecular packaging films with green synthesized AgNPs. In this study, a novel P. cocos polysaccharide (PCP) with a unique monosaccharide composition was extracted from Poria cocos (Schw.) Wolf. Then, this polysaccharide containing 24.68 % rhamnose was used as a stabilizer for the green synthesis of PCP-AgNPs for the first time. PCP-AgNPs exhibited excellent antibacterial activity against P. aeruginosa, E. coli, and S. aureus, with the highest antibacterial activity against E. coli (inhibition zone diameter = 11.14 ± 0.79 mm). Subsequently, PCP-AgNPs/chitosan (CS) film was successfully prepared by incorporating PCP-AgNPs into the CS film solution. Several experiments demonstrated that the addition of this nanomaterial promoted the formation of noncovalent interactions between CS and PCP-AgNPs, resulting in a more regular and denser film. Compared to the CS film and control group, the PCP-AgNPs/CS film significantly maintained the quality indexes of strawberries. Therefore, this composite film successfully extended the shelf life of strawberries. Regarding safety, these packaging films were not cytotoxic toward RAW264.7 cells. In conclusion, the environmentally friendly PCP-AgNPs/CS film has the potential to replace some traditional food packaging materials.
为减少传统塑料包装造成的污染并制备银纳米颗粒（AgNPs），本研究旨在开发含有绿色合成AgNPs的生物大分子包装薄膜。在本研究中，从茯苓（Poria cocos (Schw.) Wolf）中提取了一种具有独特单糖组成的新型茯苓多糖（PCP）。然后，首次将这种含有24.68%鼠李糖的多糖用作稳定剂，用于绿色合成PCP-AgNPs。PCP-AgNPs对铜绿假单胞菌、大肠杆菌和金黄色葡萄球菌表现出优异的抗菌活性，其中对大肠杆菌的抗菌活性最高（抑菌圈直径 = 11.14 ± 0.79 mm）。随后，通过将PCP-AgNPs掺入壳聚糖（CS）薄膜溶液中，成功制备了PCP-AgNPs/CS薄膜。多项实验表明，添加这种纳米材料促进了CS与PCP-AgNPs之间非共价相互作用的形成，从而形成了更规则、更致密的薄膜。与CS薄膜和对照组相比，PCP-AgNPs/CS薄膜显著保持了草莓的品质指标。因此，这种复合薄膜成功延长了草莓的货架期。在安全性方面，这些包装薄膜对RAW264.7细胞无细胞毒性。总之，环保型PCP-AgNPs/CS薄膜有潜力替代一些传统食品包装材料。
