Kim Se-Min, Kim Tai-Yong, Choi Yun-Sang, Ok Gyeongsik, Lim Min-Cheol
Research Group of Food Safety and Distribution, Korea Food Research Institute, Wanju-gun 55365, Republic of Korea.
Department of Food Science and Biotechnology, Chung-Ang University, Anseong-si 17546, Republic of Korea.
Microorganisms. 2024 Oct 18;12(10):2089. doi: 10.3390/microorganisms12102089.
In this study, silver nanoparticles (AgNPs) were synthesized using a green method from an extract of the edible insect (O_extract). The formation of AgNPs (O_AgNPs) was confirmed via UV-vis spectroscopy, and their stability was assessed using Turbiscan analysis. The size and morphology of the synthesized particles were characterized using transmission electron microscopy and field-emission scanning electron microscopy. Dynamic light scattering and zeta potential analyses further confirmed the size distribution and dispersion stability of the particles. The average particle size was 111.8 ± 1.5 nm, indicating relatively high stability. The synthesized O_AgNPs were further characterized using X-ray photoelectron spectroscopy (XPS), high-resolution X-ray diffraction (HR-XRD), and Fourier transform infrared (FTIR) spectroscopy. XPS analysis confirmed the chemical composition of the O_AgNP surface, whereas HR-XRD confirmed its crystallinity. FTIR analysis suggested that the O_extract plays a crucial role in the synthesis process. The antibacterial activity of the O_AgNPs was demonstrated using a disk diffusion assay, which revealed effective activity against common foodborne pathogens, including Typhimurium, , , and . O_AgNPs exhibited clear antibacterial activity, with inhibition zones of 15.08 ± 0.45 mm for . Typhimurium, 15.03 ± 0.15 mm for , 15.24 ± 0.66 mm for , and 13.30 ± 0.16 mm for . These findings suggest that the O_AgNPs synthesized from the O_extract have potential for use as antibacterial agents against foodborne bacteria.
在本研究中，采用绿色方法从食用昆虫提取物（O提取物）中合成了银纳米颗粒（AgNPs）。通过紫外可见光谱确认了AgNPs（O_AgNPs）的形成，并使用Turbiscan分析评估了它们的稳定性。使用透射电子显微镜和场发射扫描电子显微镜对合成颗粒的尺寸和形态进行了表征。动态光散射和zeta电位分析进一步证实了颗粒的尺寸分布和分散稳定性。平均粒径为111.8±1.5nm，表明具有较高的稳定性。使用X射线光电子能谱（XPS）、高分辨率X射线衍射（HR-XRD）和傅里叶变换红外（FTIR）光谱对合成的O_AgNPs进行了进一步表征。XPS分析确认了O_AgNP表面的化学成分，而HR-XRD确认了其结晶度。FTIR分析表明，O提取物在合成过程中起关键作用。使用纸片扩散法证明了O_AgNPs的抗菌活性，该方法显示其对常见食源性病原体具有有效活性，包括鼠伤寒沙门氏菌、[此处原文缺失三种病原体名称]。O_AgNPs表现出明显的抗菌活性，对鼠伤寒沙门氏菌的抑菌圈为15.08±0.45mm，对[此处原文缺失一种病原体名称]为15.03±0.15mm，对[此处原文缺失一种病原体名称]为15.24±0.66mm，对[此处原文缺失一种病原体名称]为13.30±0.16mm。这些发现表明，从O提取物合成的O_AgNPs有潜力用作抗食源细菌的抗菌剂。
