OBJECTIVE: To explore the knowledge, attitude, and practice (KAP) toward glioma of patients with neurological symptoms or diseases. METHODS: This web-based cross-sectional study was conducted at two medical centers in Henan Province between January 2023 and April 2023 and enrolled patients with neurological symptoms or diseases. The demographic characteristics of the participants and their KAP toward glioma were collected using a self-administered questionnaire. A structural equation modeling (SEM) was used to examine the relationship among KAP dimensions. RESULTS: The study included 442 valid questionnaires. The mean knowledge, attitude, and practice scores were 7.65 ± 1.62 (possible range: 0-9), 37.98 ± 3.17 (possible range: 9-45), and 40.16 ± 4.17 (possible range: 10-50), indicating good knowledge, favorable attitude, and active practice. The SEM analysis showed that knowledge directly affected attitudes (β = 0.89, 95%CI: 0.73-1.06, P < 0.001) but not practice (β = -0.08, 95%CI: -0.32-0.14, P = 0.487), while attitudes directly affected practice (β = 0.35, 95%CI: 0.21-0.48, P < 0.001). CONCLUSION: Patients with neurological symptoms/diseases who had heard of gliomas had good knowledge, favorable attitudes, and active practice toward glioma. Specific knowledge items that would warrant improvements were identified in the specific population of patients with neurological symptoms/diseases who had heard of glioma. Future studies should also examine the general population.