Sommai Sukruthai, Wanapat Metha, Suntara Chanon, Prachumchai Rittikeard, Cherdthong Anusorn
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
Department of Animal Science, Faculty of Agricultural Technology, Rajamangala, University of Technology Thanyaburi, Pathum Thani, 12130, Thailand.
Heliyon. 2024 Apr 20;10(9):e29972. doi: 10.1016/j.heliyon.2024.e29972. eCollection 2024 May 15.
The objective of this experiment was to study the effects of Brazilian spinach () pellet (BSP) supplementation on rumen fermentation, protozoal population, and methane (CH) estimation in beef cattle. Four male Thai native beef cattle, 3 years old, with an average bodyweight of 180 ± 5 kg, were randomly arranged in a 4 × 4 Latin square design. The cattle were supplemented (on-top) with four levels of BSP (2, 4, 6, and 8% dry matter intake (DMI), respectively). The roughage component, derived from rice straw, was fed at 40 % of DMI, while the concentrate diet was fed at 60 % of DMI. The result of the experiment demonstrated that BSP supplementation had no effect on the DMI, nutrient intake, or nutrient digestibility (p > 0.05). Rumen pH and ammonia-nitrogen concentration were not significant, while the average protozoal population linearly decreased (p = 0.002) with BSP supplementation. Mean blood urea-nitrogen concentration was linearly increased (p = 0.004) when increasing the level of BSP. Brazilian spinach pellet had no significant effect on total volatile fatty acids (TVFA), VFA profiles, and CH estimation (p > 0.05). Nitrogen balance was no different from the supplementation of BSP. The study indicates that Brazilian spinach pellet supplementation showed no noticeable effects on feed intake, rumen parameters, and nitrogen utilization; however, at 6-8% of DMI, there was a decrease in protozoal population, with no corresponding reduction in CH estimation.
本实验的目的是研究添加巴西菠菜（）颗粒（BSP）对肉牛瘤胃发酵、原生动物数量和甲烷（CH）排放估算的影响。选用4头3岁的泰国本地雄性肉牛，平均体重180±5千克，采用4×4拉丁方设计随机安排。分别给牛补充四种水平的BSP（干物质采食量（DMI）的2%、4%、6%和8%）。来自稻草的粗饲料以DMI的40%投喂，精饲料以DMI的60%投喂。实验结果表明，添加BSP对DMI、养分摄入量或养分消化率没有影响（p>0.05）。瘤胃pH值和氨氮浓度无显著差异，而添加BSP后原生动物平均数量呈线性下降（p=0.002）。随着BSP水平的提高，平均血尿素氮浓度呈线性增加（p=0.004）。巴西菠菜颗粒对总挥发性脂肪酸（TVFA）、挥发性脂肪酸谱和CH排放估算没有显著影响（p>0.05）。氮平衡与添加BSP无差异。研究表明，添加巴西菠菜颗粒对采食量、瘤胃参数和氮利用没有明显影响；然而，在DMI的6%-8%时，原生动物数量减少，但CH排放估算没有相应降低。
