Lai Jiahui, Liu Yuwei, Lu Gang, Yung Patrick, Wang Xiaoying, Tuan Rocky S, Li Zhong Alan
Department of Biomedical Engineering, The Chinese University of Hong Kong, NT, Hong Kong SAR, China.
Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, NT, Hong Kong SAR, China.
Bioact Mater. 2024 Apr 23;37:348-377. doi: 10.1016/j.bioactmat.2024.03.033. eCollection 2024 Jul.
Setting time as the fourth dimension, 4D printing allows us to construct dynamic structures that can change their shape, property, or functionality over time under stimuli, leading to a wave of innovations in various fields. Recently, 4D printing of smart biomaterials, biological components, and living cells into dynamic living 3D constructs with 4D effects has led to an exciting field of 4D bioprinting. 4D bioprinting has gained increasing attention and is being applied to create programmed and dynamic cell-laden constructs such as bone, cartilage, and vasculature. This review presents an overview on 4D bioprinting for engineering dynamic tissues and organs, followed by a discussion on the approaches, bioprinting technologies, smart biomaterials and smart design, bioink requirements, and applications. While much progress has been achieved, 4D bioprinting as a complex process is facing challenges that need to be addressed by transdisciplinary strategies to unleash the full potential of this advanced biofabrication technology. Finally, we present future perspectives on the rapidly evolving field of 4D bioprinting, in view of its potential, increasingly important roles in the development of advanced dynamic tissues for basic research, pharmaceutics, and regenerative medicine.
将时间作为第四维度,4D打印使我们能够构建动态结构,这些结构在刺激下可随时间改变其形状、性质或功能,从而在各个领域引发了一波创新浪潮。最近,将智能生物材料、生物组件和活细胞进行4D打印,制成具有4D效果的动态活体3D构建体,催生了一个令人兴奋的4D生物打印领域。4D生物打印越来越受到关注,并正被应用于创建诸如骨骼、软骨和脉管系统等程序化的、动态的、含有细胞的构建体。本文综述了用于工程化动态组织和器官的4D生物打印,随后讨论了其方法、生物打印技术、智能生物材料与智能设计、生物墨水要求及应用。尽管已取得了很大进展,但4D生物打印作为一个复杂过程,仍面临着一些挑战,需要通过跨学科策略来解决,以充分发挥这种先进生物制造技术的潜力。最后,鉴于4D生物打印在基础研究、制药和再生医学等先进动态组织开发中具有的潜力和日益重要的作用,我们阐述了这个快速发展领域的未来前景。
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