Research Centre for Built Environment, Climate, Water Technology and Digitalization, VIA University College, Horsens, Denmark.
Water Application and Technology, GRUNDFOS Holding A/S, Bjerringbro, Denmark.
Biofouling. 2024 Mar-Apr;40(3-4):262-279. doi: 10.1080/08927014.2024.2343839. Epub 2024 May 2.
In newly commissioned drinking-water polyethylene (PE) pipes, biofilm develops on the inner pipe surface. The microbial community composition from colonization to the establishment of mature biofilms is less known, including the effect on the distributed water quality. Biofilm development was followed through 1.5 years in PE-pipe side streams at two locations of a full-scale, non-chlorinated drinking-water distribution system (leaving a waterworks versus 5-6 km from a waterworks) along with inlet and outlet water quality. Mature biofilms were established after ∼8-9 months, dominated by , and (61-93% relative abundance), with a higher diversity (OTUs/Shannon Index/16S rRNA gene amplicon sequencing) in pipes in the far end of the distribution system. , and specifically (>30% of reads), dominated young (∼1.5-month-old) biofilms. Young biofilms were linked to increased microbiological counts in drinking water (HPC/ATP/qPCR), while the establishment of mature biofilms led to a drop in HPC and benefited the water quality, highlighting the importance of optimizing commissioning procedures for rapidly achieving mature and stable biofilms.
在新投入使用的饮用水用聚乙烯（PE）管道中，内壁会形成生物膜。从生物膜的初始定植到成熟阶段，其微生物群落组成及其对管网水质的影响尚不清楚。本研究通过 1.5 年的时间，在一个未加氯的全规模饮用水分配系统的两个位置（位于水厂的一侧和距离水厂 5-6km 处）的旁通管中，对生物膜的发展进行了跟踪，并对进水和出水水质进行了监测。大约 8-9 个月后，成熟生物膜形成，以 、 和 （相对丰度 61-93%）为主，分布系统末端管道中的生物膜多样性更高（OTUs/Shannon 指数/16S rRNA 基因扩增子测序）。和 ，特别是 （占读取序列的 30%以上），主导着年轻（约 1.5 个月龄）生物膜。年轻的生物膜与饮用水中微生物计数的增加有关（HPC/ATP/qPCR），而成熟生物膜的形成则导致 HPC 下降，并改善了水质，这突出表明优化调试程序以快速形成成熟稳定的生物膜的重要性。
