sp. nov., a halotolerant actinobacterium with antimicrobial activity.

A novel Gram-staining-positive actinobacterium with antimicrobial activity, designated CFH 90308, was isolated from the sediment of a salt lake in Yuncheng, Shanxi, south-western China. The isolate exhibited the highest 16S rRNA gene sequence similarities to G72, NBRC 15708 and S3-E (98.5, 98.4 and 98.2 %, respectively), and formed a separate clade with S3-E in phylogenetic trees. The strain grew at 15-40 ºC, pH 6.0-8.0 and could tolerate NaCl up to a concentration of 15 % (w/v). The whole genome of strain CFH 90308 consisted of 4.33 Mbp and the DNA G+C content was 69.6 mol%. The acyl type of the peptidoglycan was glycolyl and the whole-cell sugars were galactose and mannose. The cell-wall peptidoglycan mainly contained alanine, glycine and lysine. The menaquinones of strain CFH 90308 were MK-12, MK-13 and MK-11. Strain CFH 90308 contained anteiso-C, anteiso-C, iso-C and iso-C as the predominant fatty acids. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between CFH 90308 and the other species of the genus were found to be low (ANIb <81.3 %, dDDH <25.6 %). The secondary metabolite produced by strain CFH 90308 showed antibacterial activities against , , and methicillin-resistant . Based on genotypic, phenotypic and chemotaxonomic results, the isolate is considered to represent a novel species of the genus , for which the name sp. nov is proposed. The type strain is CFH 90308 (=DSM 105964=KCTC 49052).