从塔克拉玛干沙漠中的盐生植物中分离得到的新种。

sp. nov. isolated from a halophyte plant in the Taklamakan desert.

机构信息

Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin/College of Life Science, Tarim University, Alar 843300, PR China.

出版信息

Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004629.

Abstract

A bacterial strain, designated TRM 80801, was isolated from the in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China. Cells were Gram-stain-positive, aerobic, non-motile, short rods. Strain TRM 80801 grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl. Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801 within the genus with the highest similarities to YZYP 306 (98.97 %) and BBH6 (98.17 %), respectively. The DNA G+C content of TRM 80801 is 69.38 mol%. The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine. The acyl type of the peptidoglycan was glycolyl. Whole-cell sugars were ribose, mannose, glucose, rhamnose and galactose. The major cellular fatty acids were -C, -C and -C. The predominant menaquinones were MK-10, MK-11 and MK-12. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol. The whole-genome average nucleotide identity (ANI) value between strain TRM 80801 and YZYP 306 is 70.2 %. On the basis of the evidence presented in this study, strain TRM 80801 is representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is TRM 80801 (=CCTCC AB 2019248=KCTC 49357).

摘要

一株细菌菌株,被命名为 TRM 80801,从中国西北部新疆维吾尔自治区的塔克拉玛干沙漠中分离出来。细胞革兰氏阳性,需氧,不运动,呈短杆状。菌株 TRM 80801 在 4-50°C 下生长,最适生长温度为 28°C,在 pH 值为 6.0-11.0 和 1-15%(w/v)NaCl 下生长。16S rRNA 基因序列的系统发育分析将菌株 TRM 80801置于属内,与 YZYP 306(98.97%)和 BBH6(98.17%)的相似性最高。TRM 80801 的 DNA G+C 含量为 69.38 mol%。细胞壁肽聚糖含有鸟氨酸、谷氨酸、甘氨酸和丙氨酸,其特征二氨基酸为鸟氨酸。肽聚糖的酰基类型为甘酰基。全细胞糖为核糖、甘露糖、葡萄糖、鼠李糖和半乳糖。主要细胞脂肪酸为 -C、-C 和 -C。主要的menaquinones 为 MK-10、MK-11 和 MK-12。极性脂类为双磷脂酰甘油、磷脂酰甘油。菌株 TRM 80801 与 YZYP 306 的全基因组平均核苷酸同一性(ANI)值为 70.2%。基于本研究提供的证据,菌株 TRM 80801 代表了属内的一个新种,因此建议将其命名为 sp. nov.。模式菌株为 TRM 80801(=CCTCC AB 2019248=KCTC 49357)。

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