Wang Yuqi, Gao Mengru, Jiang Yunfeng, Huang Wuzhou, Zhao Xin, Zhu Wei, Li Hao, Wang Yi, Zeng Jian, Wu Dandan, Wei Yuming, Zhou Yonghong, Zheng Youliang, Zhang Peng, Chen Guoyue, Kang Houyang
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Theor Appl Genet. 2024 May 2;137(5):116. doi: 10.1007/s00122-024-04620-1.
An adult plant gene for resistance to stripe rust was narrowed down to the proximal one-third of the 2NS segment translocated from Aegilops ventricosa to wheat chromosome arm 2AS, and based on the gene expression analysis, two candidate genes were identified showing a stronger response at the adult plant stage compared to the seedling stage. The 2NS translocation from Aegilops ventricosa, known for its resistance to various diseases, has been pivotal in global wheat breeding for more than three decades. Here, we identified an adult plant resistance (APR) gene in the 2NS segment in wheat line K13-868. Through fine mapping in a segregating near-isogenic line (NIL) derived population of 6389 plants, the candidate region for the APR gene was narrowed down to between 19.36 Mb and 33 Mb in the Jagger reference genome. Transcriptome analysis in NILs strongly suggested that this APR gene conferred resistance to stripe rust by triggering plant innate immune responses. Based on the gene expression analysis, two disease resistance-associated genes within the candidate region, TraesJAG2A03G00588940 and TraesJAG2A03G00590140, exhibited a stronger response to Puccinia striiformis f. sp. tritici (Pst) infection at the adult plant stage than at the seedling stage, indicating that they could be potential candidates for the resistance gene. Additionally, we developed a co-dominant InDel marker, InDel_31.05, for detecting this APR gene. Applying this marker showed that over one-half of the wheat varieties approved in 2021 and 2022 in Sichuan province, China, carry this gene. Agronomic trait evaluation of NILs indicated that the 2NS segment effectively mitigated the negative effects of stripe rust on yield without affecting other important agronomic traits. This study provided valuable insights for cloning and breeding through the utilization of the APR gene present in the 2NS segment.
一个抗条锈病的成年植株基因被定位到从小麦草转移至小麦染色体臂2AS的2NS区段的近端三分之一处，并且基于基因表达分析，鉴定出两个候选基因，与幼苗期相比，它们在成年植株期表现出更强的反应。从小麦草转移而来的2NS片段以其对多种病害的抗性而闻名，三十多年来在全球小麦育种中发挥了关键作用。在此，我们在小麦品系K13 - 868的2NS区段中鉴定出一个成年植株抗性（APR）基因。通过在一个由6389株植物组成的分离近等基因系（NIL）衍生群体中进行精细定位，APR基因的候选区域在Jagger参考基因组中被缩小到19.36 Mb至33 Mb之间。NILs中的转录组分析强烈表明，该APR基因通过触发植物固有免疫反应赋予对条锈病的抗性。基于基因表达分析，候选区域内的两个抗病相关基因TraesJAG2A03G00588940和TraesJAG2A03G00590140在成年植株期对条锈菌（Pst）感染的反应比幼苗期更强，表明它们可能是抗性基因的潜在候选者。此外，我们开发了一个共显性插入缺失标记InDel_31.05来检测这个APR基因。应用这个标记表明，在中国四川省2021年和2022年审定的小麦品种中，超过一半携带这个基因。NILs的农艺性状评价表明，2NS区段有效减轻了条锈病对产量的负面影响，而不影响其他重要农艺性状。本研究为利用2NS区段中存在 的APR基因进行克隆和育种提供了有价值的见解。
