MIR-21通过肿瘤坏死因子-α调节肠道菌群分布促进溃疡性结肠炎进展。
MIR-21 regulating distribution of intestinal flora through TNF-α promotes progression of ulcerative colitis.
作者信息
Ke Yang, Liu Xueni, Niu Tao, Qiang Zhao, Gao Feng
机构信息
Gansu Provincial Maternity and Child-Care Hospital (Gansu Provincial Central Hospital), Department of Hematology and Oncology, Lanzhou, China.
PLA Rocket Force Characteristic Medical Center, Critical Care Medicine, Beijing, China.
出版信息
J Med Biochem. 2024 Apr 23;43(2):299-305. doi: 10.5937/jomb0-43320.
BACKGROUND
To study the changes in intestinal flora in patients with ulcerative colitis (UC), and to explore its correlations with micro ribonucleic acid (miR)-21 and serum tumor necrosis factor-a (TNF-α).
METHODS
A total of 150 patients with UC were selected and divided into remission group and seizure group according to the severity of disease. At the same time, 150 healthy people receiving physical examination in the hospital during the same period were selected as control group. The levels of fecal miR-21 and TNF-α in all subjects were determined via reverse transcription-polymerase chain reaction (RT-PCR). The correlation between miR-21 and TNF-α and their associations with the changes in intestinal bacteria in UC were analyzed using Pearson correlation analysis. The risk factors affecting the occurrence of UC were explored via multivariate logistic regression analysis.
背景
研究溃疡性结肠炎（UC）患者肠道菌群的变化，并探讨其与微小核糖核酸（miR）-21及血清肿瘤坏死因子-α（TNF-α）的相关性。
方法
选取150例UC患者，根据病情严重程度分为缓解组和发作组。同时，选取同期在本院进行体检的150例健康人作为对照组。采用逆转录-聚合酶链反应（RT-PCR）测定所有受试者粪便中miR-21和TNF-α的水平。采用Pearson相关性分析miR-21与TNF-α之间的相关性及其与UC肠道细菌变化的关系。通过多因素logistic回归分析探讨影响UC发生的危险因素。
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