College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200233, China.
Institute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China.
Chembiochem. 2024 Jul 2;25(13):e202400227. doi: 10.1002/cbic.202400227. Epub 2024 Jun 19.
Biomarkers are crucial physiological and pathological indicators in the host. Over the years, numerous detection methods have been developed for biomarkers, given their significant potential in various biological and biomedical applications. Among these, the detection system based on functionalized DNA origami has emerged as a promising approach due to its precise control over sensing modules, enabling sensitive, specific, and programmable biomarker detection. We summarize the advancements in biomarker detection using functionalized DNA origami, focusing on strategies for DNA origami functionalization, mechanisms of biomarker recognition, and applications in disease diagnosis and monitoring. These applications are organized into sections based on the type of biomarkers - nucleic acids, proteins, small molecules, and ions - and concludes with a discussion on the advantages and challenges associated with using functionalized DNA origami systems for biomarker detection.
生物标志物是宿主中至关重要的生理和病理指标。多年来，由于其在各种生物和生物医学应用中的巨大潜力，已经开发出了许多用于生物标志物检测的方法。在这些方法中，基于功能化 DNA 折纸的检测系统因其对传感模块的精确控制而成为一种很有前途的方法，能够实现敏感、特异和可编程的生物标志物检测。我们总结了使用功能化 DNA 折纸进行生物标志物检测的进展，重点介绍了 DNA 折纸功能化的策略、生物标志物识别的机制以及在疾病诊断和监测中的应用。这些应用根据生物标志物的类型（核酸、蛋白质、小分子和离子）进行分组，并讨论了使用功能化 DNA 折纸系统进行生物标志物检测的优点和挑战。
