Biomaterials, Drug Delivery and Nanotechnology Unit, Centre for Biomedical and Biomaterials Research, University of Mauritius, Réduit, Mauritius.
Animalerie, Plateforme de recherche CYROI, 2 rue Maxime Rivière, 97490 Sainte Clotilde, Ile De La Réunion, France.
Biomater Adv. 2024 Jul;161:213870. doi: 10.1016/j.bioadv.2024.213870. Epub 2024 Apr 28.
Wound healing following skin tumour surgery still remains a major challenge. To address this issue, polysaccharide-loaded nanofibrous mats have been engineered as skin patches on the wound site to improve wound healing while simultaneously eliminating residual cancer cells which may cause cancer relapse. The marine derived polysaccharides kappa-carrageenan (KCG) and fucoidan (FUC) were blended with polydioxanone (PDX) nanofibers due to their inherent anti-cancer activity conferred by the sulphate groups as well as their immunomodulatory properties which can reduce inflammation resulting in accelerated wound healing. KCG and FUC were released sustainably from the blend nanofibers via the Korsmeyer-Peppas kinetics. MTT assays, live/dead staining and SEM images demonstrated the toxicity of KCG and FUC towards skin cancer MP 41 cells. In addition, MP 41 cells showed reduced metastatic potential when grown on KCG or FUC containing mats. Both KCG and FUC were non- cytotoxic to healthy L 929 fibroblast cells. In vivo studies on healthy Wistar rats confirmed the non-toxicity of the nanofibrous patches as well as their improved and scarless wound healing potential. In vivo studies on tumour xenograft model further showed a reduction of 7.15 % in tumour volume in only 4 days following application of the transdermal patch.
皮肤肿瘤手术后的伤口愈合仍然是一个主要挑战。为了解决这个问题，已将负载多糖的纳米纤维垫设计为伤口部位的皮肤贴片，以改善伤口愈合，同时消除可能导致癌症复发的残留癌细胞。海洋来源的多糖角叉菜胶（KCG）和褐藻糖胶（FUC）与聚二氧杂环已酮（PDX）纳米纤维混合，因为其硫酸基团赋予的固有抗癌活性以及其免疫调节特性可以减少炎症，从而加速伤口愈合。KCG 和 FUC 通过 Korsmeyer-Peppas 动力学从共混纳米纤维中持续释放。MTT 测定、活/死染色和 SEM 图像表明 KCG 和 FUC 对皮肤癌 MP 41 细胞具有毒性。此外，当在含有 KCG 或 FUC 的垫子上生长时，MP 41 细胞的转移潜力降低。KCG 和 FUC 对健康的 L 929 成纤维细胞均无细胞毒性。在健康 Wistar 大鼠的体内研究证实了纳米纤维贴片的非毒性及其改善和无疤痕愈合的潜力。肿瘤异种移植模型的体内研究进一步表明，在应用透皮贴片仅 4 天后，肿瘤体积减少了 7.15%。
