Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, People's Republic of China.
Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, People's Republic of China; Key Laboratory of Agricultural Functional Molecule Design and Utilization of Heilongjiang Province, Harbin 150030, People's Republic of China.
Sci Total Environ. 2024 Jun 25;931:172866. doi: 10.1016/j.scitotenv.2024.172866. Epub 2024 May 4.
Tetracycline antibiotics (TCs) are extensively used in clinical medicine, animal husbandry, and aquaculture because of their cost-effectiveness and high antibacterial efficacy. However, the presence of TCs residues in the environment poses risks to humans. In this study, an inner filter effect (IFE) fluorescent probe, 2,2'-(ethane-1,2-diylbis((2-((2-methylquinolin-8-yl)amino)-2-oxoethyl)azanediyl))diacetic acid (MQDA), was developed for the rapid detection of Eu within 30 s. And its complex [MQDA-Eu] was successfully used for the detection of TCs. Upon coordination of a carboxyl of MQDA with Eu to form a [MQDA-Eu] complex, the carboxyl served as an antenna ligand for the effective detection of Eu to intensify the emission intensity of MQDA via "antenna effect", the process was the energy absorbed by TCs via UV excitation was effectively transferred to Eu. Fluorescence quenching of the [MQDA-Eu] complex was caused by the IFE in multicolor fluorescence systems. The limits of detection of [MQDA-Eu] for oxytetracycline, chlorotetracycline hydrochloride, and tetracycline were 0.80, 0.93, and 1.7 μM in DMSO/HEPES (7:3, v/v, pH = 7.0), respectively. [MQDA-Eu] demonstrated sensitive detection of TCs in environmental and food samples with satisfactory recoveries and exhibited excellent imaging capabilities for TCs in living cells and zebrafish with low cytotoxicity. The proposed approach demonstrated considerable potential for the quantitative detection of TCs.
四环素类抗生素（TCs）由于其成本效益高和高效抗菌作用，广泛应用于临床医学、畜牧业和水产养殖。然而，TCs 残留存在于环境中会对人类健康构成风险。在本研究中，开发了一种内滤效应（IFE）荧光探针，2,2'-(乙烷-1,2-二基双((2-((2-甲基喹啉-8-基)氨基)-2-氧代乙基)亚氨基)二乙酸（MQDA），用于在 30 秒内快速检测 Eu。并成功地将其配合物[MQDA-Eu]用于检测 TCs。MQDA 的一个羧基与 Eu 配位形成[MQDA-Eu]配合物，该羧基作为天线配体，通过“天线效应”有效增强了 MQDA 的发射强度，该过程是 TCs 通过 UV 激发吸收的能量有效地转移到 Eu 上。多色荧光体系中的 IFE 导致 [MQDA-Eu] 配合物的荧光猝灭。在 DMSO/HEPES（7:3，v/v，pH=7.0）中，[MQDA-Eu] 对土霉素、盐酸金霉素和四环素的检测限分别为 0.80、0.93 和 1.7μM。[MQDA-Eu] 对环境和食品样品中的 TCs 具有灵敏的检测能力，回收率令人满意，并且对活细胞和斑马鱼中的 TCs 具有出色的成像能力，细胞毒性低。该方法为 TCs 的定量检测提供了有价值的方法。
