Zhang Xingping, Feng Yujun, Chen Xiaowei, Wang Yuanyuan, Guo Yingli, Yang Xin, Yang Hualin
Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, 434025, China.
Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, China.
Mikrochim Acta. 2025 Jun 3;192(6):394. doi: 10.1007/s00604-025-07265-z.
A sensitive fluorescence and colorimetric dual-mode detection method based on Eu and FeO@MoS nanocomposites was constructed. In this technique, tetracycline antibiotics (TCs) chelated with Eu to form a Eu-TCs complex which emits red fluorescence at 620 nm through the antenna effect. At the same time, Eu-TCs complex enhances the peroxidase-like activity of FeO@MoS to catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) that can produce a colorimetric signal. This proposed strategy has been used to detect three antibiotics including oxytetracycline (OTC), tetracycline (TC), and chlortetracycline hydrochloride (HCl-CTC). The linear ranges of the new assay for OTC, TC, and HCl-CTC were 0.1-25 μM, 0.5-25 μM, and 0.05-5 μM with the detection limits of 0.016 μM, 0.039 μM, and 0.036 μM, respectively. Meanwhile, this new method was fast and convenient which can complete the detection in 22 min. The assay has been effectively applied to TCs detection in tap water, lake water and honey with satisfactory recoveries of 90.55-109.86%.
构建了一种基于铕（Eu）和FeO@MoS纳米复合材料的灵敏荧光和比色双模式检测方法。在该技术中，四环素类抗生素（TCs）与Eu螯合形成Eu-TCs络合物，该络合物通过天线效应在620 nm处发射红色荧光。同时，Eu-TCs络合物增强了FeO@MoS的过氧化物酶样活性，以催化3,3',5,5'-四甲基联苯胺（TMB）的氧化，从而产生比色信号。该方法已用于检测三种抗生素，包括土霉素（OTC）、四环素（TC）和盐酸金霉素（HCl-CTC）。新方法对OTC、TC和HCl-CTC的线性范围分别为0.1 - 25 μM、0.5 - 25 μM和0.05 - 5 μM，检测限分别为0.016 μM、0.039 μM和0.036 μM。同时，该新方法快速便捷，可在22分钟内完成检测。该方法已有效地应用于自来水、湖水和蜂蜜中TCs的检测，回收率为90.55 - 109.86%，结果令人满意。
