Matthews N
Br J Cancer. 1979 Oct;40(4):534-9. doi: 10.1038/bjc.1979.218.
Tumour-necrosis factor (TNF) is growth-inhibitory or cytotoxic to certain tumour cell lines, and is present in the serum of rabbits injected i.v. with BCG and endotoxin 2 weeks apart (TNF serum). TNF serum also has interferon activity, and as TNF and interferons have a number of properties in common their relationship has been investigated further. TNF was assayed by cytotoxicity in vitro against L cells and interferon by a CPE-inhibition assay with Semliki Forest virus.TNF appears not to be an interferon, on the following bases:1. TNF activity could be separated from the Type I interferon of TNF serum by passage through a Cibacron blue-agarose column or by sequential salt precipitation, ion-exchange chromatography and gel filtration.2. Preparations of Type I interferon induced by poly I, poly C or virus lacked TNF activity.3. Though it was not possible to compare TNF with rabbit Type II interferon (as methods used to induce Type II interferon in other species were unsuccessful in the rabbit) rabbit TNF has a number of properties which distinguish it from the Type II interferons of other species.4. Rabbit TNF inhibited the growth of a human melanoma cell line, and also had effects on certain mouse and rabbit cell lines, whereas the anti-cellular effects of interferons are reported to be species-specific.
肿瘤坏死因子（TNF）对某些肿瘤细胞系具有生长抑制作用或细胞毒性，并且存在于间隔两周静脉注射卡介苗和内毒素的兔子血清中（TNF血清）。TNF血清也具有干扰素活性，由于TNF和干扰素具有许多共同特性，因此对它们之间的关系进行了进一步研究。通过体外对L细胞的细胞毒性测定TNF，通过用Semliki森林病毒进行的CPE抑制试验测定干扰素。基于以下依据，TNF似乎不是一种干扰素：1. 通过Cibacron蓝琼脂糖柱或通过连续盐沉淀、离子交换色谱和凝胶过滤，可将TNF血清中的I型干扰素与TNF活性分离。2. 由聚I、聚C或病毒诱导的I型干扰素制剂缺乏TNF活性。3. 虽然无法将TNF与兔II型干扰素进行比较（因为用于在其他物种中诱导II型干扰素的方法在兔子中未成功），但兔TNF具有许多特性，使其与其他物种的II型干扰素区分开来。4. 兔TNF抑制人黑色素瘤细胞系的生长，并且对某些小鼠和兔细胞系也有作用，而据报道干扰素的抗细胞作用具有种属特异性。
