Department of Medicine, Hematology and Oncology, UK Knappschaftskrankenhaus Bochum GmbH, Ruhr University of Bochum, Bochum, Germany.
Department of Hematology and Oncology, Evangelisches Krankenhaus Hamm, Hamm, Germany.
J Clin Apher. 2024 Jun;39(3):e22114. doi: 10.1002/jca.22114.
Stem cell apheresis in the context of autologous stem cell transplantation requires an accurate cluster of differentiantion 34 (CD34) count determined by flow cytometry as the current gold standard. Since flow cytometry is a personnel and time-intensive diagnostic tool, automated stem cell enumeration may provide a promising alternative. Hence, this study aimed to compare automated hematopoietic progenitor enumeration carried out on a Sysmex XN-20 module compared with conventional flow cytometric measurements.
One hundred forty-three blood samples from 41 patients were included in this study. Correlation between the two methods was calculated over all samples, depending on leukocyte count and diagnosis.
Overall, we found a high degree of correlation (r = 0.884). Furthermore, correlation was not impaired by elevated leukocyte counts (>10 000/μL, r = 0.860 vs <10 000/μL, r = 0.849; >20 000/μL, r = 0.843 vs <20 000/μL, r = 0.875). However, correlation was significantly impaired in patients with multiple myeloma (multiple myeloma r = 0.840 vs nonmyeloma r = 0.934).
Stem cell measurement carried out on the Sysmex XN-20 module provides a significant correlation with flow cytometry and might be implemented in clinical practice. In clinical decision-making, there was discrepancy of under 15% of cases. In multiple myeloma patients, XN-20 should be used with caution.
在自体干细胞移植的背景下，干细胞吸取出需要通过流式细胞术确定准确的分化簇 34（CD34）计数，这是当前的金标准。由于流式细胞术是一种人员和时间密集型的诊断工具，自动化干细胞计数可能提供一种有前途的替代方法。因此，本研究旨在比较在 Sysmex XN-20 模块上进行的自动化造血祖细胞计数与传统流式细胞术测量。
这项研究纳入了 41 名患者的 143 份血液样本。计算了两种方法在所有样本上的相关性，取决于白细胞计数和诊断。
总体而言，我们发现高度相关性（r=0.884）。此外，相关性不受白细胞计数升高（>10,000/μL，r=0.860 与 <10,000/μL，r=0.849；>20,000/μL，r=0.843 与 <20,000/μL，r=0.875）的影响。然而，在多发性骨髓瘤患者中，相关性显著受损（多发性骨髓瘤 r=0.840 与非骨髓瘤 r=0.934）。
在 Sysmex XN-20 模块上进行的干细胞测量与流式细胞术具有显著相关性，可能在临床实践中得到实施。在临床决策中，有 15%以下的病例存在差异。在多发性骨髓瘤患者中，应谨慎使用 XN-20。
