Nauss K M, Pavlina T M, Kumar V, Newberne P M
Gastroenterology. 1984 Mar;86(3):468-75.
Using successive ethylenediaminetetraacetic acid and collagenase treatments, two fractions of mucosal lymphocytes have been isolated from the rat large intestine that differ in morphologic and functional characteristics. Intraepithelial lymphocytes consisted largely of granular lymphocytes (91 +/- 6%) that did not respond to stimulation with phytohemagglutinin or concanavalin A, but had natural killer cytotoxic activity against the YAC-1 cell line. The natural killer cytotoxicity of the intraepithelial lymphocytes was specifically reduced by the addition of increasing numbers of unlabeled homologous tumor cells but not by unlabeled thymocytes. The sensitivity of different target cell lines to lysis by intraepithelial lymphocytes was the same as splenocytes from the same rat strain. Lymphocytes from the lamina propria contained 21 +/- 4% granular cells with the remainder being typical small lymphocytes. The lamina propria fraction responded well to stimulation with concanavalin A, phytohemagglutinin, and pokeweek mitogen, and also had natural killer activity against YAC-1 cells.
通过连续的乙二胺四乙酸和胶原酶处理，从大鼠大肠中分离出了两部分黏膜淋巴细胞，它们在形态和功能特征上有所不同。上皮内淋巴细胞主要由颗粒淋巴细胞组成（91±6%），这些细胞对植物血凝素或刀豆球蛋白A的刺激无反应，但对YAC-1细胞系具有自然杀伤细胞毒性活性。添加越来越多未标记的同源肿瘤细胞可特异性降低上皮内淋巴细胞的自然杀伤细胞毒性，但未标记的胸腺细胞则无此作用。不同靶细胞系对上皮内淋巴细胞裂解的敏感性与同一大鼠品系的脾细胞相同。固有层淋巴细胞含有21±4%的颗粒细胞，其余为典型的小淋巴细胞。固有层部分对刀豆球蛋白A、植物血凝素和商陆有丝分裂原的刺激反应良好，并且对YAC-1细胞也具有自然杀伤活性。
