鉴定 2 型糖尿病小鼠腮腺中 circRNAs 的表达谱和功能网络。
Identification of circRNAs expression profiles and functional networks in parotid gland of type 2 diabetes mouse.
机构信息
Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials, 100081, Beijing, P.R. China.
Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Xiamen Medical College, Xiamen Key Laboratory of Stomotalogical Disease Diagnosis and Treatment, 361006, Xiamen, P.R. China.
出版信息
BMC Genomics. 2024 May 7;25(1):450. doi: 10.1186/s12864-024-10290-6.
BACKGROUND
Circular RNAs (circRNAs) are a novel kind of non-coding RNAs proved to play crucial roles in the development of multiple diabetic complications. However, their expression and function in diabetes mellitus (DM)-impaired salivary glands are unknown.
RESULTS
By using microarray technology, 663 upregulated and 999 downregulated circRNAs companied with 813 upregulated and 525 downregulated mRNAs were identified in the parotid glands (PGs) of type2 DM mice under a 2-fold change and P < 0.05 cutoff criteria. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analysis of upregulated mRNAs showed enrichments in immune system process and peroxisome proliferator-activated receptor (PPAR) signaling pathway. Infiltration of inflammatory cells and increased inflammatory cytokines were observed in diabetic PGs. Seven differently expressed circRNAs validated by qRT-PCR were selected for coding-non-coding gene co-expression (CNC) and competing endogenous RNA (ceRNA) networks analysis. PPAR signaling pathway was primarily enriched through analysis of circRNA-mRNA networks. Moreover, the circRNA-miRNA-mRNA networks highlighted an enrichment in the regulation of actin cytoskeleton.
CONCLUSION
The inflammatory response is elevated in diabetic PGs. The selected seven distinct circRNAs may attribute to the injury of diabetic PG by modulating inflammatory response through PPAR signaling pathway and actin cytoskeleton in diabetic PGs.
背景
环状 RNA（circRNAs）是一种新型的非编码 RNA，已被证明在多种糖尿病并发症的发生发展中发挥着关键作用。然而，它们在糖尿病（DM）损伤的唾液腺中的表达和功能尚不清楚。
结果
通过使用微阵列技术，在 2 倍变化和 P < 0.05 截止标准下，在 2 型 DM 小鼠的腮腺（PG）中鉴定出 663 个上调和 999 个下调的 circRNAs 以及 813 个上调和 525 个下调的 mRNA。上调 mRNA 的基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析显示，它们在免疫系统过程和过氧化物酶体增殖物激活受体（PPAR）信号通路中富集。在糖尿病 PG 中观察到炎症细胞浸润和炎症细胞因子增加。通过 qRT-PCR 验证的 7 个差异表达的 circRNAs 被选择用于编码-非编码基因共表达（CNC）和竞争内源性 RNA（ceRNA）网络分析。通过 circRNA-mRNA 网络分析，PPAR 信号通路主要富集。此外，circRNA-miRNA-mRNA 网络强调了对肌动蛋白细胞骨架调节的富集。
结论
糖尿病 PG 中的炎症反应增强。所选的 7 个不同的 circRNAs 可能通过调节 PPAR 信号通路和糖尿病 PG 中的肌动蛋白细胞骨架，导致糖尿病 PG 的损伤。
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