Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Wuxi, Jiangsu, 214081, China.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, Jiangsu, 214081, China.
BMC Genomics. 2024 May 7;25(1):446. doi: 10.1186/s12864-024-10327-w.
Air exposure is an inevitable source of stress that leads to significant mortality in Coilia nasus. Our previous research demonstrated that adding 10‰ NaCl to aquatic water could enhance survival rates, albeit the molecular mechanisms involved in air exposure and salinity mitigation remained unclear. Conversely, salinity mitigation resulted in decreased plasma glucose levels and improved antioxidative activity. To shed light on this phenomenon, we characterized the transcriptomic changes in the C. nasus brain upon air exposure and salinity mitigation by integrated miRNA-mRNA analysis.
The plasma glucose level was elevated during air exposure, whereas it decreased during salinity mitigation. Antioxidant activity was suppressed during air exposure, but was enhanced during salinity mitigation. A total of 629 differentially expressed miRNAs (DEMs) and 791 differentially expressed genes (DEGs) were detected during air exposure, while 429 DEMs and 1016 DEGs were identified during salinity mitigation. GO analysis revealed that the target genes of DEMs and DEGs were enriched in biological process and cellular component during air exposure and salinity mitigation. KEGG analysis revealed that the target genes of DEMs and DEGs were enriched in metabolism. Integrated analysis showed that 24 and 36 predicted miRNA-mRNA regulatory pairs participating in regulating glucose metabolism, Ca transport, inflammation, and oxidative stress. Interestingly, most of these miRNAs were novel miRNAs.
In this study, substantial miRNA-mRNA regulation pairs were predicted via integrated analysis of small RNA sequencing and RNA-Seq. Based on predicted miRNA-mRNA regulation and potential function of DEGs, miRNA-mRNA regulatory network involved in glucose metabolism and Ca transport, inflammation, and oxidative stress in C. nasus brain during air exposure and salinity mitigation. They regulated the increased/decreased plasma glucose and inhibited/promoted antioxidant activity during air exposure and salinity mitigation. Our findings would propose novel insights to the mechanisms underlying fish responses to air exposure and salinity mitigation.
空气暴露是导致鲥鱼死亡率显著增加的不可避免的应激源。我们之前的研究表明，在水中添加 10‰的 NaCl 可以提高存活率，尽管涉及空气暴露和盐度缓解的分子机制尚不清楚。相反，盐度缓解会导致血浆葡萄糖水平降低，抗氧化活性提高。为了阐明这一现象，我们通过整合 miRNA-mRNA 分析，研究了鲥鱼脑在空气暴露和盐度缓解时的转录组变化。
空气暴露时血浆葡萄糖水平升高，盐度缓解时血浆葡萄糖水平降低。空气暴露时抗氧化活性受到抑制，盐度缓解时抗氧化活性增强。空气暴露时共检测到 629 个差异表达 miRNA（DEM）和 791 个差异表达基因（DEG），盐度缓解时检测到 429 个 DEM 和 1016 个 DEG。GO 分析显示，空气暴露和盐度缓解时，DEM 和 DEG 的靶基因富集在生物过程和细胞组成中。KEGG 分析显示，DEM 和 DEG 的靶基因富集在代谢途径中。整合分析显示，有 24 个和 36 个预测的 miRNA-mRNA 调控对参与调节葡萄糖代谢、Ca 转运、炎症和氧化应激。有趣的是，这些 miRNA 大多是新的 miRNA。
本研究通过对小 RNA 测序和 RNA-Seq 的综合分析，预测了大量的 miRNA-mRNA 调控对。基于预测的 miRNA-mRNA 调控和 DEGs 的潜在功能，构建了鲥鱼脑在空气暴露和盐度缓解时参与葡萄糖代谢和 Ca 转运、炎症和氧化应激的 miRNA-mRNA 调控网络。它们调节了空气暴露和盐度缓解时血浆葡萄糖的增加/减少，并抑制/促进了抗氧化活性。我们的研究结果为鱼类对空气暴露和盐度缓解的反应机制提供了新的见解。
