体外对分泌单克隆免疫球蛋白M抗绵羊红细胞抗体的慢性淋巴细胞白血病淋巴细胞的独特型抑制

In vitro idiotypic suppression of chronic lymphocytic leukemia lymphocytes secreting monoclonal immunoglobulin M anti-sheep erythrocyte antibody.

作者信息

Bona C A, Fauci A S

出版信息

J Clin Invest. 1980 Mar;65(3):761-7. doi: 10.1172/JCI109724.

DOI:10.1172/JCI109724

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC371420/

Abstract

A patient with chronic lymphocytic leukemia was found to have B cells with surface immunoglobulin (Ig)M manifesting anti-sheep erythrocyte (SRBC) specificity together with a high titer serum monoclonal anti-SRBC IgM antibody. By immunizing a sheep with the monoclonal IgM antibody, followed by multiple absorptions against normal human IgM, an anti-idiotype (Id) antibody was obtained. The serum IgM anti-SRBC antibody was then demonstrated to share the same idiotypic determinants with the surface IgM with anti-SRBC specificity on the patient's B cells. The anti-Id antibody suppressed the spontaneous secretion of anti-SRBC antibody as well as the pokeweed mitogen-induced anti-SRBC antibody production as measured by a hemolysis-in-gel plaque-forming cell assay. In contrast, pokeweed mitogen-induced anti-SRBC plaque-forming cell responses of normal individuals were not suppressed by the anti-Id antibody. Thus, this study demonstrates in vitro suppression of human B-cell function by anti-Id antibody.

摘要

一名慢性淋巴细胞白血病患者被发现其带有表面免疫球蛋白（Ig）M的B细胞表现出抗绵羊红细胞（SRBC）特异性，同时伴有高滴度血清单克隆抗SRBC IgM抗体。用该单克隆IgM抗体免疫一只绵羊，随后多次吸收正常人IgM，获得了一种抗独特型（Id）抗体。结果证明，血清IgM抗SRBC抗体与患者B细胞上具有抗SRBC特异性的表面IgM具有相同的独特型决定簇。通过凝胶溶血空斑形成细胞试验检测，抗Id抗体抑制了抗SRBC抗体的自发分泌以及商陆有丝分裂原诱导的抗SRBC抗体产生。相比之下，抗Id抗体并未抑制正常人的商陆有丝分裂原诱导的抗SRBC空斑形成细胞反应。因此，本研究证明了抗Id抗体在体外对人B细胞功能的抑制作用。

相似文献

1

In vitro idiotypic suppression of chronic lymphocytic leukemia lymphocytes secreting monoclonal immunoglobulin M anti-sheep erythrocyte antibody.体外对分泌单克隆免疫球蛋白M抗绵羊红细胞抗体的慢性淋巴细胞白血病淋巴细胞的独特型抑制

J Clin Invest. 1980 Mar;65(3):761-7. doi: 10.1172/JCI109724.

2

In vitro interaction with antigen suppresses the differentiation of chronic lymphocytic leukemia cells secreting monoclonal anti-sheep red blood cell antibody.与抗原的体外相互作用抑制了分泌单克隆抗绵羊红细胞抗体的慢性淋巴细胞白血病细胞的分化。

J Immunol. 1983 Oct;131(4):2046-9.

3

B-cell malignancy and monoclonal gammopathy, and idiotype of cell surface and serum immunoglobulin.B细胞恶性肿瘤和单克隆丙种球蛋白病，以及细胞表面和血清免疫球蛋白的独特型。

Jpn J Clin Oncol. 1983 Sep;13(3):533-42.

4

Circulating monoclonal IgM proteins in B cell chronic lymphocytic leukemia: their identification, characterization and relationship to membrane IgM.B 细胞慢性淋巴细胞白血病中的循环单克隆 IgM 蛋白：其鉴定、特征及其与膜 IgM 的关系。

J Immunol. 1984 Dec;133(6):3396-400.

5

Anti-immunoglobulin autoantibodies are not preferentially induced in polyclonal activation of human and mouse lymphocytes, and more anti-DNA and anti-erythrocyte autoantibodies are induced in polyclonal activation of mouse than human lymphocytes.抗免疫球蛋白自身抗体在人和小鼠淋巴细胞的多克隆激活中并非优先被诱导产生，并且在小鼠淋巴细胞的多克隆激活中比在人淋巴细胞的多克隆激活中诱导产生更多的抗DNA和抗红细胞自身抗体。

J Immunol. 1984 Nov;133(5):2537-44.

6

Activation of human B lymphocytes. XIV. Characterization of the precursor of the pokeweed mitogen-induced anti-sheep red blood cell plaque-forming cell.人B淋巴细胞的激活。十四。商陆有丝分裂原诱导的抗绵羊红细胞空斑形成细胞前体的特性

J Immunol. 1979 Aug;123(2):890-5.

7

"Nonspecific" immunoenhancing T cells in tumor-bearing mice include anti-idiotypic subsets.荷瘤小鼠中的“非特异性”免疫增强T细胞包括抗独特型亚群。

J Immunol. 1983 Nov;131(5):2576-9.

8

Idiotypes on B lymphocytes: association with immunoglobulins.B淋巴细胞上的独特型：与免疫球蛋白的关联

J Immunol. 1983 Apr;130(4):1819-25.

9

Activation of human B lymphocytes. XV. Spontaneously occurring and mitogen-induced indirect anti-sheep red blood cell plaque-forming cells in normal human peripheral blood.人类B淋巴细胞的激活。十五、正常人外周血中自发产生的和丝裂原诱导的间接抗绵羊红细胞空斑形成细胞

J Immunol. 1980 May;124(5):2410-3.

10

Immunization with idiotypic immunoglobulin protects against development of B lymphocytic leukemia, but emerging tumor cells can evade antibody attack by modulation.用独特型免疫球蛋白进行免疫可预防B淋巴细胞白血病的发生，但新出现的肿瘤细胞可通过调节作用逃避抗体攻击。

J Immunol. 1983 Feb;130(2):970-3.

引用本文的文献

1

Spontaneous in vitro differentiation of antigen-specific lymphocytes from a patient with immunoglobulin M gammopathy.一名患有免疫球蛋白M型丙种球蛋白病患者的抗原特异性淋巴细胞的自发体外分化。

J Clin Invest. 1982 Dec;70(6):1292-9. doi: 10.1172/jci110728.

2

Cellular localization of rheumatoid factor idiotypes.类风湿因子独特型的细胞定位。

J Clin Invest. 1982 Jun;69(6):1356-65. doi: 10.1172/jci110575.

3

Suppression of in vitro monoclonal human rheumatoid factor synthesis by antiidiotypic antibody. Target cells and molecular requirements.抗独特型抗体对体外人单克隆类风湿因子合成的抑制作用。靶细胞和分子要求。

J Clin Invest. 1983 Oct;72(4):1410-9. doi: 10.1172/JCI111097.

4

Regulation of immunoglobulin E antibody synthesis in man by antiidiotypic antibodies.抗独特型抗体对人免疫球蛋白E抗体合成的调节

J Clin Invest. 1983 Jan;71(1):46-54. doi: 10.1172/jci110750.

5

Consumption of monoclonal anti-idiotypic antibody by neoplastic B lymphocytes: a guide for immunotherapy.肿瘤性B淋巴细胞对单克隆抗独特型抗体的摄取：免疫治疗指南

Br J Cancer. 1984 Sep;50(3):407-13. doi: 10.1038/bjc.1984.190.

6

Paraproteinaemia: use of idiotypic determinants as specific markers for tumour activity.副蛋白血症：将独特型决定簇用作肿瘤活性的特异性标志物。

Clin Exp Immunol. 1981 Sep;45(3):544-51.

7

Phenotypic and functional analysis of lymphocytes in myasthenia gravis.重症肌无力患者淋巴细胞的表型与功能分析

Springer Semin Immunopathol. 1985;8(3):209-33. doi: 10.1007/BF00197297.

本文引用的文献

1

Surface bound immunoglobulins as a cell marker in human lymphoproliferative diseases.表面结合免疫球蛋白作为人类淋巴增殖性疾病中的细胞标志物

Blood. 1972 Dec;40(6):777-94.

2

Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody.通过给予抗独特型抗体抑制成年小鼠的独特型特异性。

J Exp Med. 1972 Jun 1;135(6):1293-300. doi: 10.1084/jem.135.6.1293.

3

Idiotypic specificity of surface immunoglobulin and the maturation of leukemic bone-marrow-derived lymphocytes.表面免疫球蛋白的独特型特异性与白血病骨髓来源淋巴细胞的成熟

Proc Natl Acad Sci U S A. 1974 Nov;71(11):4487-90. doi: 10.1073/pnas.71.11.4487.

4

Occurrence of surface IgM, IgD, and free light chains of human lymphocytes.人类淋巴细胞表面免疫球蛋白M、免疫球蛋白D和游离轻链的出现情况。

J Exp Med. 1974 Feb 1;139(2):451-6. doi: 10.1084/jem.139.2.451.

5

B and T cell markers in human proliferative blood diseases and primary immunodeficiencies, with special reference to membrane bound immunoglobulins.人类增殖性血液疾病和原发性免疫缺陷中的B细胞和T细胞标志物，特别提及膜结合免疫球蛋白。

Transplant Rev. 1973;16:85-113. doi: 10.1111/j.1600-065x.1973.tb00118.x.

6

Biologically active water-insoluble protein polymers. I. Their use for isolation of antigens and antibodies.生物活性水不溶性蛋白质聚合物。I. 其在抗原和抗体分离中的应用。

J Biol Chem. 1967 Apr 10;242(7):1651-9.

7

Regulation of immune response by autogenous antibody against receptor.自身抗受体抗体对免疫反应的调节

Proc Natl Acad Sci U S A. 1974 Dec;71(12):5083-7. doi: 10.1073/pnas.71.12.5083.

8

Myeloma proteins as tumor-specific transplantation antigens.骨髓瘤蛋白作为肿瘤特异性移植抗原。

Proc Natl Acad Sci U S A. 1972 Jun;69(6):1540-4. doi: 10.1073/pnas.69.6.1540.

9

Human bone marrow lymphocytes. I. Distribution of lymphocyte subpopulations in the bone marrow of normal individuals.人骨髓淋巴细胞。I. 正常个体骨髓中淋巴细胞亚群的分布。

J Clin Invest. 1975 Jul;56(1):98-110. doi: 10.1172/JCI108085.

10

Release from maternally-induced allotypic suppression in rabbit by Nocardia water-soluble mitogen.诺卡氏菌水溶性有丝分裂原使兔从母体诱导的同种异型抑制中释放出来。

J Exp Med. 1977 Sep 1;146(3):881-6. doi: 10.1084/jem.146.3.881.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。