• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

无细胞生产平台上用于酰基辅酶A前体的时间分辨多参数分析

Time-resolved multiparameter analytics on a cell-free production platform for acyl-CoA precursors.

作者信息

Maehler Dominic, Hoefgen Sandra, Münchberg Ute, Schmitz Oliver J, Rautschek Julia, Huang Ying, Freier Erik, Valiante Vito

机构信息

CARS Microscopy Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Dortmund Germany.

Biobricks of Microbial Natural Product Syntheses Leibniz Institute for Natural Product Research and Infections Biology - Hans Knöll Institute Jena Germany.

出版信息

Anal Sci Adv. 2022 Oct 18;3(11-12):289-296. doi: 10.1002/ansa.202200021. eCollection 2022 Dec.

DOI:10.1002/ansa.202200021
PMID:38715841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10989585/
Abstract

Cell-free biosynthesis is emerging as a very attractive alternative for the production of market-relevant molecules. The free combination of enzymes, regardless of where they are isolated from, raises the possibility to build more efficient synthetic routes but at the same time leads to higher complexity regarding the analysis of the different enzymatic steps. Here we present an analytical method for the real-time analysis of acyl-CoA blocks forming and consuming during multi-step catalyses. We focused on malonyl-Coenzyme A and acetyl-CoA, which are the most used acyl-CoA units for carbon chain elongations. By employing capillary electrophoresis, we could detect the decrease of educts and the formation of products in a time-resolved fashion.

摘要

无细胞生物合成正成为生产与市场相关分子的一种极具吸引力的替代方法。酶的自由组合，无论它们是从何处分离得到的，都增加了构建更高效合成路线的可能性，但同时也导致了分析不同酶促步骤的复杂性增加。在此，我们提出了一种用于实时分析多步催化过程中酰基辅酶A的形成和消耗的分析方法。我们聚焦于丙二酰辅酶A和乙酰辅酶A，它们是碳链延长过程中最常用的酰基辅酶A单元。通过采用毛细管电泳，我们能够以时间分辨的方式检测反应物的减少和产物的形成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/06c55e46a27e/ANSA-3-289-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/209c580cdc1f/ANSA-3-289-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/1577b6466be0/ANSA-3-289-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/e113681408a0/ANSA-3-289-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/06c55e46a27e/ANSA-3-289-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/209c580cdc1f/ANSA-3-289-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/1577b6466be0/ANSA-3-289-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/e113681408a0/ANSA-3-289-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7d7/10989585/06c55e46a27e/ANSA-3-289-g004.jpg

相似文献

1
Time-resolved multiparameter analytics on a cell-free production platform for acyl-CoA precursors.无细胞生产平台上用于酰基辅酶A前体的时间分辨多参数分析
Anal Sci Adv. 2022 Oct 18;3(11-12):289-296. doi: 10.1002/ansa.202200021. eCollection 2022 Dec.
2
AccR, a TetR Family Transcriptional Repressor, Coordinates Short-Chain Acyl Coenzyme A Homeostasis in .AccR，一种 TetR 家族转录阻遏物，协调. 中的短链酰基辅酶 A 稳态。
Appl Environ Microbiol. 2020 Jun 2;86(12). doi: 10.1128/AEM.00508-20.
3
Enzymes involved in fatty acid and polyketide biosynthesis in Streptomyces glaucescens: role of FabH and FabD and their acyl carrier protein specificity.参与浅绿链霉菌脂肪酸和聚酮化合物生物合成的酶：FabH和FabD的作用及其酰基载体蛋白特异性
Biochemistry. 2002 Aug 20;41(33):10462-71. doi: 10.1021/bi0258804.
4
Streptomyces coelicolor RedP and FabH enzymes, initiating undecylprodiginine and fatty acid biosynthesis, exhibit distinct acyl-CoA and malonyl-acyl carrier protein substrate specificities.链霉菌红色 P 和 FabH 酶，启动十一碳烯基丙二酰基辅酶 A 和脂肪酸生物合成，表现出不同的酰基辅酶 A 和丙二酰基酰基载体蛋白的底物特异性。
FEMS Microbiol Lett. 2012 Mar;328(1):32-8. doi: 10.1111/j.1574-6968.2011.02474.x. Epub 2012 Jan 4.
5
Regulation of plant Fatty Acid biosynthesis : analysis of acyl-coenzyme a and acyl-acyl carrier protein substrate pools in spinach and pea chloroplasts.植物脂肪酸生物合成的调控：菠菜和豌豆叶绿体中酰基辅酶A和酰基-酰基载体蛋白底物库的分析
Plant Physiol. 1992 Oct;100(2):923-30. doi: 10.1104/pp.100.2.923.
6
Fatty acid biosynthesis in Erlich cells. The mechanism of short term control by exogenous free fatty acids.艾氏腹水癌细胞中的脂肪酸生物合成。外源性游离脂肪酸的短期调控机制。
J Biol Chem. 1975 Jul 25;250(14):5419-25.
7
Improving fatty acid production in Escherichia coli through the overexpression of malonyl coA-acyl carrier protein transacylase.通过过表达丙二酰辅酶 A-酰基载体蛋白转酰基酶提高大肠杆菌中的脂肪酸产量。
Biotechnol Prog. 2012 Jan-Feb;28(1):60-5. doi: 10.1002/btpr.716. Epub 2011 Oct 28.
8
Substrate specificity of chalcone synthase from Petroselinum hortense. Formation of phloroglucinol derivatives from aliphatic substrates.园芹查尔酮合酶的底物特异性。由脂肪族底物形成间苯三酚衍生物。
J Biol Chem. 1983 Jun 10;258(11):6730-4.
9
The R117A variant of the Escherichia coli transacylase FabD synthesizes novel acyl-(acyl carrier proteins).大肠杆菌转酰基酶 FabD 的 R117A 变体合成新型酰基-(酰基载体蛋白)。
Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8431-8441. doi: 10.1007/s00253-017-8586-9. Epub 2017 Oct 26.
10
Involvement of long-chain acyl coenzyme A for lipid synthesis in repression of acetyl-coenzyme A carboxylase in Candida lipolytica.长链脂酰辅酶A参与解脂假丝酵母中乙酰辅酶A羧化酶的抑制作用以进行脂质合成。
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4390-4. doi: 10.1073/pnas.76.9.4390.

本文引用的文献

1
Intrinsic Ability of the β-Oxidation Pathway To Produce Bioactive Styrylpyrones.β-氧化途径产生生物活性苯乙烯基吡喃酮的内在能力。
Angew Chem Int Ed Engl. 2022 Aug 22;61(34):e202206851. doi: 10.1002/anie.202206851. Epub 2022 Jul 13.
2
A Single LC-MS/MS Analysis to Quantify CoA Biosynthetic Intermediates and Short-Chain Acyl CoAs.用于定量辅酶A生物合成中间体和短链酰基辅酶A的单重液相色谱-串联质谱分析
Metabolites. 2021 Jul 21;11(8):468. doi: 10.3390/metabo11080468.
3
Rational Design of Flavonoid Production Routes Using Combinatorial and Precursor-Directed Biosynthesis.
利用组合生物合成和前体导向生物合成对类黄酮生产途径进行合理设计
ACS Synth Biol. 2020 Jul 17;9(7):1823-1832. doi: 10.1021/acssynbio.0c00172. Epub 2020 Jun 27.
4
Some thoughts about enantioseparations in capillary electrophoresis.关于毛细管电泳对映体拆分的一些思考。
Electrophoresis. 2019 Sep;40(18-19):2420-2437. doi: 10.1002/elps.201900144. Epub 2019 May 21.
5
Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design.通过酶设计构建从一碳到乙酰辅酶 A 的合成途径。
Nat Commun. 2019 Mar 26;10(1):1378. doi: 10.1038/s41467-019-09095-z.
6
Capillary electrophoresis integrated immobilized enzyme reactor for kinetic and inhibition assays of β-secretase as the Alzheimer's disease drug target.毛细管电泳集成固定化酶反应器用于阿尔茨海默病药物靶点β-分泌酶的动力学和抑制分析。
J Sep Sci. 2019 Mar;42(5):1067-1076. doi: 10.1002/jssc.201800947. Epub 2019 Feb 4.
7
Recent advances in capillary electrophoresis-mass spectrometry: Instrumentation, methodology and applications.毛细管电泳-质谱联用技术的最新进展：仪器、方法学及应用。
Electrophoresis. 2019 Jan;40(1):79-112. doi: 10.1002/elps.201800331. Epub 2018 Nov 12.
8
Advances in enzyme substrate analysis with capillary electrophoresis.毛细管电泳中酶底物分析的进展。
Methods. 2018 Aug 15;146:93-106. doi: 10.1016/j.ymeth.2018.02.005. Epub 2018 Feb 27.
9
Enzymatic Production of Glutathione Coupling with an ATP Regeneration System Based on Polyphosphate Kinase.基于多聚磷酸激酶的谷胱甘肽与 ATP 再生系统的偶联的酶法生产。
Appl Biochem Biotechnol. 2018 Jun;185(2):385-395. doi: 10.1007/s12010-017-2664-4. Epub 2017 Nov 21.
10
Artificial Biocatalytic Linear Cascades for Preparation of Organic Molecules.人工生物催化线性级联反应在有机分子制备中的应用。
Chem Rev. 2018 Jan 10;118(1):270-348. doi: 10.1021/acs.chemrev.7b00033. Epub 2017 May 8.