Krakauer T, Oppenheim J J, Jasin H E
Cell Immunol. 1985 Mar;91(1):92-9. doi: 10.1016/0008-8749(85)90034-6.
Human monocyte factors mediate cartilage matrix degradation by activation of the resident chondrocytes. In the present work, the cartilage matrix-degrading activity of partially purified human monocyte-derived interleukin 1 has been investigated. Human monocyte or blood mononuclear cell culture supernatants were sequentially purified by phenyl-Sepharose and gel filtration chromatography, or by gel filtration chromatography, isoelectric focusing, or chromatofocusing. Column fractions were simultaneously tested by the standard method that defines interleukin 1 activity--costimulation of mouse thymocyte proliferation--and for matrix macromolecule release from living bovine cartilage explants in organ culture. The two activities showed identical profiles in purification steps that would discriminate according to molecular size, hydrophobicity, and net charge. Moreover, the thermal denaturation profiles of the material purified by chromatofocusing could not distinguish between thymocyte proliferating and matrix degrading activities. These results suggest that interleukin 1 which is present in inflammatory synovial fluids may play an important role in the mediation of cartilage damage in chronic inflammatory arthritides.
人类单核细胞因子通过激活驻留软骨细胞介导软骨基质降解。在本研究中，已对部分纯化的人单核细胞衍生白细胞介素1的软骨基质降解活性进行了研究。人单核细胞或血液单核细胞培养上清液依次通过苯基琼脂糖和凝胶过滤色谱法，或通过凝胶过滤色谱法、等电聚焦或色谱聚焦进行纯化。柱级分通过定义白细胞介素1活性的标准方法（即共刺激小鼠胸腺细胞增殖）以及从器官培养中的活牛软骨外植体释放基质大分子进行同时测试。在根据分子大小、疏水性和净电荷进行区分的纯化步骤中，这两种活性表现出相同的谱图。此外，通过色谱聚焦纯化的物质的热变性谱图无法区分胸腺细胞增殖活性和基质降解活性。这些结果表明，存在于炎性滑液中的白细胞介素1可能在慢性炎性关节炎的软骨损伤介导中起重要作用。
