从人滑膜中部分纯化出一种具有白细胞介素1、软骨细胞刺激活性和类分解素活性的因子。

Partial purification of a factor from human synovium that possesses interleukin 1, chondrocyte stimulating and catabolin-like activities.

作者信息

Elford P R, Meats J E, Sharrard R M, Russell R G

出版信息

FEBS Lett. 1985 Jan 7;179(2):247-51. doi: 10.1016/0014-5793(85)80528-7.

Abstract

Human synovial explants in culture release material that stimulates the production of prostaglandin E2 (PGE2) and several extracellular enzymes by human chondrocytes. Fractionation of conditioned medium by gel filtration revealed a protein of approx. 15 kDa, which in addition to stimulating production of PGE2 and plasminogen activator by human articular chondrocytes, possessed interleukin 1 activity and induced cartilage degradation. Further purification using iso-electric focussing again showed co-elution of these activities with a major pI of 6.9 and a minor pI of 5.1-5.3. This study indicated that human synovium releases a factor that is closely related to or identical with interleukin 1 and suggests that this protein may participate in cellular interactions that occur within the rheumatoid joint.

摘要

培养中的人滑膜外植体释放出一种物质，该物质可刺激人软骨细胞产生前列腺素E2（PGE2）和几种细胞外酶。通过凝胶过滤对条件培养基进行分级分离，发现了一种约15 kDa的蛋白质，该蛋白质除了刺激人关节软骨细胞产生PGE2和纤溶酶原激活剂外，还具有白细胞介素1活性并诱导软骨降解。使用等电聚焦进一步纯化再次表明，这些活性与主要pI为6.9和次要pI为5.1 - 5.3共同洗脱。这项研究表明，人滑膜释放出一种与白细胞介素1密切相关或相同的因子，并表明该蛋白质可能参与类风湿性关节内发生的细胞相互作用。