从人滑膜中部分纯化出一种具有白细胞介素1、软骨细胞刺激活性和类分解素活性的因子。
Partial purification of a factor from human synovium that possesses interleukin 1, chondrocyte stimulating and catabolin-like activities.
作者信息
Elford P R, Meats J E, Sharrard R M, Russell R G
出版信息
FEBS Lett. 1985 Jan 7;179(2):247-51. doi: 10.1016/0014-5793(85)80528-7.
Human synovial explants in culture release material that stimulates the production of prostaglandin E2 (PGE2) and several extracellular enzymes by human chondrocytes. Fractionation of conditioned medium by gel filtration revealed a protein of approx. 15 kDa, which in addition to stimulating production of PGE2 and plasminogen activator by human articular chondrocytes, possessed interleukin 1 activity and induced cartilage degradation. Further purification using iso-electric focussing again showed co-elution of these activities with a major pI of 6.9 and a minor pI of 5.1-5.3. This study indicated that human synovium releases a factor that is closely related to or identical with interleukin 1 and suggests that this protein may participate in cellular interactions that occur within the rheumatoid joint.
培养中的人滑膜外植体释放出一种物质，该物质可刺激人软骨细胞产生前列腺素E2（PGE2）和几种细胞外酶。通过凝胶过滤对条件培养基进行分级分离，发现了一种约15 kDa的蛋白质，该蛋白质除了刺激人关节软骨细胞产生PGE2和纤溶酶原激活剂外，还具有白细胞介素1活性并诱导软骨降解。使用等电聚焦进一步纯化再次表明，这些活性与主要pI为6.9和次要pI为5.1 - 5.3共同洗脱。这项研究表明，人滑膜释放出一种与白细胞介素1密切相关或相同的因子，并表明该蛋白质可能参与类风湿性关节内发生的细胞相互作用。
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