Rawle F C, Platts-Mills T A, Mitchell E B
Clin Exp Immunol. 1985 Jan;59(1):101-9.
We have recently shown that peripheral blood mononuclear cells from patients sensitive to the house dust mite, D. pteronyssinus, will proliferate in vitro in response to the purified major allergen, antigen P1. Such cell populations, separated on Ficoll gradients, were shown to contain basophils, and had an average histamine content of 12 ng/10(6) cells. Incubation with antigen P1 resulted in the release of histamine, and histamine is known to activate T suppressor cells. In the present experiments we observed up to 80% inhibition of proliferation (mean 50-60%) with histamine added at 3.3 X 10(-7)-3.3 X 10(-5) M. Cultures of T cells supplemented with irradiated non-T cells, that had been depleted of cells bearing surface membrane IgG, IgM and IgE, contained on average 63% less histamine than unseparated cultures. However, no consistent difference in the proliferative response to antigen P1 was observed. Depletion of histamine by pre-incubation of the cells with antigen P1 at 10(-3)-10(-4) micrograms/ml followed by washing of the cells before culture also produced no significant change in the proliferative response. Passage of cell population over nylon wool resulted in depletion of basophils, as well as other cell types, and generally led to a decrease in proliferation. We conclude that release of mediators from basophils in cell cultures does not markedly affect the magnitude of the proliferative response to antigen P1. The varied responses seen with cells from different individuals are likely to reflect differences in the numbers of circulating allergen sensitized T cells.
我们最近发现，对屋尘螨（粉尘螨）敏感的患者外周血单核细胞，在体外会对纯化的主要变应原抗原P1产生增殖反应。经Ficoll梯度分离的此类细胞群体显示含有嗜碱性粒细胞，其组胺平均含量为12 ng/10⁶个细胞。与抗原P1孵育导致组胺释放，且已知组胺可激活抑制性T细胞。在本实验中，我们观察到添加浓度为3.3×10⁻⁷ - 3.3×10⁻⁵ M的组胺时，增殖抑制高达80%（平均50 - 60%）。补充了经辐照的非T细胞（已去除表面膜IgG、IgM和IgE的细胞）的T细胞培养物，其组胺含量平均比未分离的培养物少63%。然而，在对抗原P1的增殖反应中未观察到一致的差异。在培养前用10⁻³ - 10⁻⁴ μg/ml的抗原P1对细胞进行预孵育，然后洗涤细胞以去除组胺，这也未使增殖反应产生显著变化。细胞群体通过尼龙毛柱后，嗜碱性粒细胞以及其他细胞类型减少，通常导致增殖下降。我们得出结论，细胞培养中嗜碱性粒细胞释放介质不会显著影响对抗原P1的增殖反应强度。不同个体细胞出现的不同反应可能反映了循环中变应原致敏T细胞数量的差异。
