Rocklin R E, Haberek-Davidson A
J Clin Immunol. 1981 Jan;1(1):73-9. doi: 10.1007/BF00915479.
Aliquots of human blood mononuclear cells were cultured in vitro in the absence or presence of varying concentrations of histamine (10(-3)-10(-8) M) for 24 hr, mitomycin treated, washed, and cocultured with autologous indicator cells that were then stimulated by PHA. The degree of suppression of thymidine uptake was measured in the presence of histamine-activated cells. The results demonstrated that histamine, in a dose-dependent fashion (10(-3) and 10(-4) M), significantly suppressed the PHA proliferative response. A minimum of 2 hr was required to activate cells by histamine to express suppressor function. While initial studies were carried out with a 1:1 suppressor:indicator cell ratio, it was found that maximum suppression resulted with lower ratios (1:2 or 1:5) in some experiments, and was dependent somewhat on the concentration of histamine used. That the suppressor cells expressed histamine receptors was shown by the finding that histamine-Sepharose columns (but not control columns) depleted the histamine-reactive cells. In addition, Con A-reactive suppressor cells also adhered to histamine columns. Based on experiments with H1 and H2 antagonists and agonists, the histamine-responsive cell was apparently activated through its histamine type-2 receptor.
将人血单核细胞的等分试样在不存在或存在不同浓度组胺（10⁻³ - 10⁻⁸ M）的情况下体外培养24小时，用丝裂霉素处理，洗涤，并与自体指示细胞共培养，然后用PHA刺激指示细胞。在存在组胺激活细胞的情况下测量胸苷摄取的抑制程度。结果表明，组胺以剂量依赖方式（10⁻³和10⁻⁴ M）显著抑制PHA增殖反应。组胺激活细胞以表达抑制功能至少需要2小时。虽然最初的研究是以1:1的抑制细胞：指示细胞比例进行的，但发现在某些实验中较低比例（1:2或1:5）产生最大抑制，并且在一定程度上取决于所用组胺的浓度。组胺 - 琼脂糖柱（而非对照柱）耗尽组胺反应性细胞这一发现表明抑制细胞表达组胺受体。此外，Con A反应性抑制细胞也粘附于组胺柱。基于使用H1和H2拮抗剂及激动剂的实验，组胺反应性细胞显然是通过其组胺2型受体被激活的。
