Londei M, Bottazzo G F, Feldmann M
Science. 1985 Apr 5;228(4695):85-9. doi: 10.1126/science.3871967.
The thyroid glands of patients with autoimmune diseases such as Graves' disease and certain forms of goiter contain infiltrating activated T lymphocytes and, unlike cells of normal glands, the epithelial follicular cells strongly express histocompatibility antigens of the HLA-DR type. In a study of such autoimmune disorders, the infiltrating T cells from the thyroid glands of two patients with Graves' disease were cloned in mitogen-free interleukin-2 (T-cell growth factor). The clones were expanded and their specificity was tested. Three types of clones were found. One group, of T4 phenotype, specifically recognized autologous thyroid cells. Another, also of T4 phenotype, recognized autologous thyroid or blood cells and thus responded positively in the autologous mixed lymphocyte reaction. Other clones derived from cells that were activated in vivo were of no known specificity. These clones provide a model of a human autoimmune disease and their analysis should clarify mechanisms of pathogenesis and provide clues to abrogating these undesirable immune responses.
患有自身免疫性疾病（如格雷夫斯病和某些类型的甲状腺肿）的患者的甲状腺含有浸润性活化T淋巴细胞，与正常甲状腺细胞不同，上皮滤泡细胞强烈表达HLA - DR型组织相容性抗原。在一项针对此类自身免疫性疾病的研究中，从两名格雷夫斯病患者的甲状腺中提取的浸润性T细胞在无丝裂原的白细胞介素-2（T细胞生长因子）中进行克隆。这些克隆被扩增并测试其特异性。发现了三种类型的克隆。一组为T4表型，特异性识别自体甲状腺细胞。另一组同样为T4表型，识别自体甲状腺或血细胞，因此在自体混合淋巴细胞反应中呈阳性反应。其他源自体内活化细胞的克隆具有未知特异性。这些克隆提供了一种人类自身免疫性疾病模型，对它们的分析应能阐明发病机制，并为消除这些不良免疫反应提供线索。
