基于上转换磷光技术的侧向流动分析快速检测
Rapid Detection of by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay.
机构信息
College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, China.
Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun, China.
出版信息
Front Cell Infect Microbiol. 2021 Nov 10;11:762472. doi: 10.3389/fcimb.2021.762472. eCollection 2021.
Cysticercosis is a neglected tropical disease caused by the larvae of in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of . UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.
囊尾蚴病是一种由猪和人寄生虫幼虫引起的被忽视的热带病。目前，猪囊尾蚴病的诊断较为困难，传统的病理学检测无法满足检测需求。本研究建立了一种用于检测 的 UPT-LF 检测方法。UCP 颗粒与两种抗原，TSOL18 和 GP50 结合；通过生物传感器将样品捕获，将 UCP 颗粒的信号转换为可检测的信号进行分析。与 ELISA 相比，UPT-LF 具有更高的灵敏度和特异性，TSOL18 和 GP50 抗原的灵敏度分别为 93.59%和 97.44%，特异性均为 100%。鉴于其快速、小体积、高灵敏度和特异性以及良好的稳定性和重现性，该方法可用于囊尾蚴病的诊断。
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