Murray L J, Dhut S, Walker L C, Rainey M, Habeshaw J A
Clin Exp Immunol. 1985 Feb;59(2):315-26.
Monoclonal antibodies were raised against a laboratory derived variant of the Raji Burkitt lymphoma cell line. We have characterized two of these antibodies by screening on haematopoietic cell lines, and frozen sections of both reactive and neoplastic lymphoid tissue, and found reactivity with separate cellular components of lymphoid follicles. Monoclonal FW37.4.D5 reacts in section specifically with follicular dendritic reticular cells. Monoclonal 35.1C5 reacts with a subpopulation of splenic marginal zone, and lymph node mantle zone, B lymphocytes, but stains only rare cells within germinal centres. This monoclonal is operationally B lymphocyte specific, distinguishing an 'intermediate' B cell subset, represented in lymphoma by B chronic lymphocytic leukaemia, B hairy cell leukaemia and centrocytic lymphoma.
制备了针对拉吉伯基特淋巴瘤细胞系实验室衍生变体的单克隆抗体。我们通过对造血细胞系以及反应性和肿瘤性淋巴组织的冰冻切片进行筛选，对其中两种抗体进行了表征，发现它们与淋巴滤泡的不同细胞成分发生反应。单克隆抗体FW37.4.D5在切片中特异性地与滤泡树突状网状细胞发生反应。单克隆抗体35.1C5与脾边缘区和淋巴结套区的B淋巴细胞亚群发生反应，但仅对生发中心内的罕见细胞染色。这种单克隆抗体在操作上具有B淋巴细胞特异性，可区分一个“中间”B细胞亚群，在淋巴瘤中由B慢性淋巴细胞白血病、B毛细胞白血病和中心细胞淋巴瘤代表。
