Johnson G D, Hardie D L, Ling N R, Maclennan I C
Clin Exp Immunol. 1986 Apr;64(1):205-13.
A collection of new and established FDC-reactive MoAb has been used in an immunohistological study designed to throw light on (a) the nature of FDC (which are of unknown lineage) as judged by their sharing of antigens with other cell types and (b) the reason for the strong expression of some B cell antigens on FDC. The MoAb were tested on: (1) sections of tonsil, (2) sections of lymph nodes from four cases of non-Hodgkin's lymphoma, (3) peripheral blood cells and (4) cells of cultured haemopoietic cell lines. Only one of the ten new MoAb bound to FDC and no other component of the tissues screened. It resembled R4/23, a MoAb known to be specific for FDC. The other nine antibodies showed a range of cross-reactivity patterns involving one or more of the following: monocytes, macrophages, platelets, epithelium, endothelium and connective tissue fibres. Some of the MoAb reacted with B lymphocytes and cells of B lymphoblastoid lines but none showed the restricted FDC-staining pattern associated with MoAb which detect the CD23, P45 antigen. The findings are discussed in terms of the intrinsic or extrinsic nature of the antigens detected.
一组新的和已有的FDC反应性单克隆抗体被用于一项免疫组织学研究，该研究旨在阐明：(a) 根据FDC（其谱系未知）与其他细胞类型共享抗原的情况来判断其性质，以及(b) FDC上某些B细胞抗原强烈表达的原因。这些单克隆抗体在以下样本上进行了测试：(1) 扁桃体切片，(2) 4例非霍奇金淋巴瘤患者的淋巴结切片，(3) 外周血细胞，以及(4) 培养的造血细胞系细胞。十种新单克隆抗体中只有一种与FDC结合，且不与所筛查组织的其他成分结合。它类似于R4/23，一种已知对FDC具有特异性的单克隆抗体。其他九种抗体表现出一系列交叉反应模式，涉及以下一种或多种细胞：单核细胞、巨噬细胞、血小板、上皮细胞、内皮细胞和结缔组织纤维。一些单克隆抗体与B淋巴细胞和B淋巴母细胞系细胞发生反应，但没有一种显示出与检测CD23、P45抗原的单克隆抗体相关的局限于FDC的染色模式。根据所检测抗原的内在或外在性质对研究结果进行了讨论。
