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1
The origin of T cell colonies from T depleted human lymphocyte populations: analysis by limiting dilution.来自T细胞耗竭的人淋巴细胞群体的T细胞集落起源：通过有限稀释法进行分析。
Clin Exp Immunol. 1985 Feb;59(2):482-90.
2
Bone marrow T cell colony-forming cells: studies of their origin and use in monitoring T cell-depleted bone marrow grafts.骨髓T细胞集落形成细胞：其起源及在监测T细胞去除的骨髓移植中的应用研究
Clin Exp Immunol. 1985 Dec;62(3):561-9.
3
Phytohemagglutinin-induced human T-lymphocyte colonies: cellular and T-cell growth factor requirements are the same as for suspension culture growth.植物血凝素诱导的人T淋巴细胞集落：细胞和T细胞生长因子需求与悬浮培养生长相同。
Nat Immun Cell Growth Regul. 1983;3(2):102-12.
4
Phenotypic and functional heterogeneity of human peripheral blood T lymphocytes producing colony stimulating factor. A clonal and precursor frequency analysis.产生集落刺激因子的人外周血T淋巴细胞的表型和功能异质性。克隆及前体细胞频率分析。
J Immunol. 1985 Dec;135(6):3933-7.
5
The role of interleukin-2 in T colony formation by human pre-T cells (pTCFC).白细胞介素-2在人前T细胞形成T集落（pTCFC）中的作用。
Clin Exp Immunol. 1984 Oct;58(1):154-60.
6
Activation through CD3 molecule leads to clonal expansion of all human peripheral blood T lymphocytes: functional analysis of clonally expanded cells.通过CD3分子激活可导致所有人外周血T淋巴细胞的克隆性扩增：对克隆性扩增细胞的功能分析。
J Immunol. 1985 Oct;135(4):2337-42.
7
Control of human T-colony formation by interleukin-2.白细胞介素-2对人T细胞集落形成的调控
Immunology. 1985 Feb;54(2):249-53.
8
Origin of T lymphocyte colony-forming cells in cell populations depleted of sheep erythrocyte rosette forming cells.绵羊红细胞花环形成细胞缺失的细胞群体中T淋巴细胞集落形成细胞的起源
Clin Exp Immunol. 1988 Jul;73(1):46-50.
9
Growth at limiting dilution of human T cell colonies from T cell-depleted peripheral blood leukocytes.来自T细胞耗竭外周血白细胞的人T细胞集落的有限稀释培养生长情况。
J Immunol. 1983 Mar;130(3):1139-45.
10
Origin of T-lymphocytes in human mixed hematopoietic colonies.人混合造血集落中T淋巴细胞的起源
Exp Hematol. 1987 Nov;15(10):1080-5.

本文引用的文献

1
Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis.用于确定免疫活性细胞频率的有限稀释分析。I. 数据分析。
J Immunol. 1981 Apr;126(4):1614-9.
2
Limit-dilution assay and clonal expansion of all T cells capable of proliferation.有限稀释分析及所有能够增殖的T细胞的克隆扩增。
J Immunol Methods. 1982 Aug 13;52(3):307-22. doi: 10.1016/0022-1759(82)90003-5.
3
Nature and mechanisms of action of co-operating cells controlling human T-colony formation.控制人类T细胞集落形成的协同细胞的性质及作用机制。
Immunology. 1982 Feb;45(2):265-71.
4
Comparison of the colony-forming capacities of human T-lymphocyte subpopulations.人类T淋巴细胞亚群集落形成能力的比较。
Clin Immunol Immunopathol. 1981 Dec;21(3):289-94. doi: 10.1016/0090-1229(81)90217-8.
5
Existence of a pool of T-lymphocyte colony-forming cells (T-CFC) in human bone marrow and their place in the differentiation of the T-lymphocyte lineage.人骨髓中T淋巴细胞集落形成细胞（T-CFC）池的存在及其在T淋巴细胞谱系分化中的地位。
Blood. 1981 Nov;58(5):911-5.
6
Human T-lymphocyte colonies: generation of colonies in different lymphocyte subpopulations.人T淋巴细胞集落：不同淋巴细胞亚群中集落的生成
Immunology. 1981 May;43(1):39-45.
7
Characterization of the T lymphocyte colony-forming cells and evidence for the acquisition of T cell markers in the absence of the thymic microenvironment in man.
J Immunol. 1981 May;126(5):2020-3.
8
Human T cell colony formation in microculture: analysis of growth requirements and functional activities.
J Immunol. 1981 Mar;126(3):1134-9.
9
A characterization of T lymphocyte colony-forming cells (TL-CFC) in human bone marrow.人骨髓中T淋巴细胞集落形成细胞（TL-CFC）的特征
Clin Exp Immunol. 1980 Sep;41(3):541-6.
10
Differentiation of precursor T lymphocytes in man and delineation of the selective abnormalities in severe combined immune deficiency disease.人类前体T淋巴细胞的分化以及严重联合免疫缺陷病中选择性异常的描述。
Clin Immunol Immunopathol. 1982 Dec;25(3):303-15. doi: 10.1016/0090-1229(82)90195-7.

来自T细胞耗竭的人淋巴细胞群体的T细胞集落起源：通过有限稀释法进行分析。

The origin of T cell colonies from T depleted human lymphocyte populations: analysis by limiting dilution.

作者信息

Lederman H M, Lee J W, Gelfand E W

出版信息

Clin Exp Immunol. 1985 Feb;59(2):482-90.

PMID:3872192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1577134/
Abstract

We have examined the identity of the T colony progenitor cell (TCPC) in T cell depleted peripheral blood mononuclear cells (PBM-E-). PBM were cultured in a limiting dilution assay utilizing PHA, T cell growth factor (TCGF) and irradiated feeder cells in semi-solid medium. The TCPC frequency was 0.10 in PBM-E+ and 0.0006 in PBM-E-. The observed TCPC frequency in PBM-E- was directly proportional to the number of contaminating PBM-E+; all of the T colonies which arose from PBM-E- could be accounted for by contaminating T cells. In other experiments, E+ cells were generated from PBM-E-, incubated in the presence of PHA and TCGF. This appearance of E+ cells could be totally abrogated by incubation with hydroxyurea. Similarly, the TCPC frequency in PBM-E- was increased by pre-incubation with PHA and TCGF, and this increase was blocked by hydroxyurea. These experiments suggest that a small number of contaminating T cells can rapidly expand in culture and that these residual T cells, not a population of pre-T cells, are the source of TCPC within the PBM-E- population.

摘要

我们已经检测了T细胞耗竭的外周血单个核细胞（PBM-E-）中T集落祖细胞（TCPC）的特性。在半固体培养基中，利用植物血凝素（PHA）、T细胞生长因子（TCGF）和经照射的饲养细胞，对PBM进行有限稀释分析培养。PBM-E+中TCPC频率为0.10，而PBM-E-中为0.0006。在PBM-E-中观察到的TCPC频率与污染的PBM-E+数量成正比；所有源自PBM-E-的T集落都可由污染的T细胞解释。在其他实验中，从PBM-E-中产生E+细胞，在PHA和TCGF存在下孵育。用羟基脲孵育可完全消除E+细胞的这种出现。同样，PBM-E-中的TCPC频率通过用PHA和TCGF预孵育而增加，且这种增加被羟基脲阻断。这些实验表明，少量污染的T细胞在培养中可迅速扩增，并且这些残留的T细胞而非前T细胞群体，是PBM-E-群体中TCPC的来源。