Koc University, İstanbul, Türkiye.
Training and Research Center, Kütahya Health Sciences University, Kutahya, Türkiye.
J Cell Mol Med. 2024 May;28(9):e18316. doi: 10.1111/jcmm.18316.
Tissue engineering includes the construction of tissue-organ scaffold. The advantage of three-dimensional scaffolds over two-dimensional scaffolds is that they provide homeostasis for a longer time. The microbial community in Symbiotic culture of bacteria and yeast (SCOBY) can be a source for kombucha (kombu tea) production. In this study, it was aimed to investigate the usage of SCOBY, which produces bacterial cellulose, as a biomaterial and 3D scaffold material. 3D printable biomaterial was obtained by partial hydrolysis of oolong tea and black tea kombucha biofilms. In order to investigate the usage of 3D kombucha biomaterial as a tissue scaffold, "L929 cell line 3D cell culture" was created and cell viability was tested in the biomaterial. At the end of the 21st day, black tea showed 51% and oolong tea 73% viability. The cytotoxicity of the materials prepared by lyophilizing oolong and black tea kombucha beverages in fibroblast cell culture was determined. Black tea IC value: 7.53 mg, oolong tea IC value is found as 6.05 mg. Fibroblast viability in 3D biomaterial + lyophilized oolong and black tea kombucha beverages, which were created using the amounts determined to these values, were investigated by cell culture Fibroblasts in lyophilized and 3D biomaterial showed viability of 58% in black tea and 78% in oolong tea at the end of the 7th day. In SEM analysis, it was concluded that fibroblast cells created adhesion to the biomaterial. 3D biomaterial from kombucha mushroom culture can be used as tissue scaffold and biomaterial.
组织工程包括组织器官支架的构建。与二维支架相比，三维支架的优势在于它们能提供更长时间的内环境稳定。共生细菌和酵母（SCOBY）微生物群落可以作为 kombucha（康普茶）生产的来源。在这项研究中，旨在研究产生细菌纤维素的 SCOBY 作为生物材料和 3D 支架材料的用途。通过乌龙茶和红茶 kombucha 生物膜的部分水解获得了 3D 可打印生物材料。为了研究 3D kombucha 生物材料作为组织支架的用途，创建了“L929 细胞系 3D 细胞培养”，并在生物材料中测试了细胞活力。在第 21 天结束时，红茶显示出 51%的活力，乌龙茶显示出 73%的活力。通过在成纤维细胞培养中冻干乌龙茶和红茶 kombucha 饮料来确定材料的细胞毒性。红茶 IC 值为 7.53mg，乌龙茶 IC 值为 6.05mg。通过细胞培养研究了使用这些值确定的量来创建的 3D 生物材料+冻干乌龙茶和红茶 kombucha 饮料中的成纤维细胞活力。在第 7 天结束时，冻干和 3D 生物材料中的成纤维细胞显示出红茶 58%和乌龙茶 78%的活力。在 SEM 分析中，得出结论，成纤维细胞在生物材料上形成了附着。来自 kombucha 蘑菇培养的 3D 生物材料可用作组织支架和生物材料。
