Paul C, Shalala K, Warren R, Smith R
Infect Immun. 1985 Apr;48(1):40-3. doi: 10.1128/iai.48.1.40-43.1985.
A spent medium antigen was prepared from the avirulent RIA strain of Salmonella typhimurium. Lymph node cells isolated from female BALB/c mice injected subcutaneously with the spent medium antigen exhibited antigen-specific proliferation. By using these cells and T-cell growth factor, continuous spent medium antigen-specific, Thy 1.2-sensitive lines were generated. These cells exhibited antigen-specific proliferation in vitro and were effective in inducing significant (P less than 0.01) host protection when adoptively transferred to naive syngeneic mice.
一种用过的培养基抗原是由鼠伤寒沙门氏菌无毒RIA菌株制备的。从皮下注射用过的培养基抗原的雌性BALB/c小鼠分离的淋巴结细胞表现出抗原特异性增殖。通过使用这些细胞和T细胞生长因子,产生了连续的用过的培养基抗原特异性、Thy 1.2敏感细胞系。这些细胞在体外表现出抗原特异性增殖,当将其过继转移到同基因的未致敏小鼠时,能有效诱导显著的(P小于0.01)宿主保护。