Bigley N J, Kreps D P, Smith R A, Esa A
Infect Immun. 1981 Apr;32(1):353-63. doi: 10.1128/iai.32.1.353-363.1981.
To assess the separate contributions of host T cells and the physical state of the antigen in the development of effective. Salmonella resistance, glutaraldehyde-treated and untreated protein- and ribonucleic acid-rich extracts (E-RNA extracts) of virulent Salmonella typhimurium SR-11 or attenuated S. typhimurium RIA were used to immunize Salmonella-resistant Salmonella-susceptible strains of mice for the purpose of determining whether antigen-specific T-cell or B-cell responses were formed and, if so, which responses predominated. The resistance imparted to each mouse strain after vaccination with S. typhimurium RIA was used as the standard for comparison. The inbred mouse strains C57BL/6 and DBA/2 and their F(1) hybrid (strain BDF(1)), outbred ICR Swiss mice, and endotoxin-resistant C3H/HeJ mice were examined for the capacity to develop resistance to lethal Salmonella infections, as well as the ability to generate antigen-reactive T cells. Only the BDF(1), C3H/HeJ, and ICR Swiss mice were able to develop resistance to challenge infections mediated by the virulent SR-11 strain of S. typhimurium after vaccination with the living, attenuated RIA strain of S. typhimurium or immunization with E-RNA extracts. We developed an assay to identify the antigen-reactive rosette-forming lymphocytes present in lymph nodes and spleens of immunized mice. Levels of 0.2% or higher of theta antigen-bearing, antigen-reactive rosette-forming cells were found in the lymph nodes or spleens or both of only the BDF(1), C3H/HeJ, and ICR Swiss mice (i.e., in the "Salmonella responder" strains). Mouse strains C57BL/6 and DBA/2, which failed to develop resistance to lethal infections after immunization with the S. typhimurium RIA vaccine or with the E-RNA extracts, lacked effective numbers of antitheta antigen-sensitive rosette-forming cells. Modification of the effective E-RNA extracts by polymerization with glutaraldehyde resulted in a marked diminution in their abilities to induce resistance to salmonellosis in the two responder mouse strains tested (BDF(1) and ICR Swiss), even though detectable levels of antibody were induced.
为评估宿主T细胞和抗原物理状态在有效沙门氏菌抗性形成中的单独作用，我们使用戊二醛处理和未处理的富含蛋白质和核糖核酸的提取物（E-RNA提取物），这些提取物来自强毒鼠伤寒沙门氏菌SR-11或减毒鼠伤寒沙门氏菌RIA，用于免疫对沙门氏菌有抗性和易感性的小鼠品系，目的是确定是否形成了抗原特异性T细胞或B细胞应答，如果形成了，哪种应答占主导。用鼠伤寒沙门氏菌RIA疫苗接种后赋予每个小鼠品系的抗性用作比较标准。对近交小鼠品系C57BL/6和DBA/2及其F(1)杂种（品系BDF(1)）、远交ICR瑞士小鼠和对内毒素有抗性的C3H/HeJ小鼠进行了检测，以评估它们产生对致死性沙门氏菌感染的抗性的能力，以及产生抗原反应性T细胞的能力。在用减毒活的鼠伤寒沙门氏菌RIA菌株接种疫苗或用E-RNA提取物免疫后，只有BDF(1)、C3H/HeJ和ICR瑞士小鼠能够对强毒鼠伤寒沙门氏菌SR-11菌株介导的攻击感染产生抗性。我们开发了一种检测方法来鉴定免疫小鼠的淋巴结和脾脏中存在的抗原反应性玫瑰花结形成淋巴细胞。仅在BDF(1)、C3H/HeJ和ICR瑞士小鼠（即“沙门氏菌应答者”品系）的淋巴结或脾脏或两者中发现了含θ抗原、抗原反应性玫瑰花结形成细胞的水平达到0.2%或更高。在用鼠伤寒沙门氏菌RIA疫苗或E-RNA提取物免疫后未能对致死性感染产生抗性的小鼠品系C57BL/6和DBA/2，缺乏有效数量的抗θ抗原敏感玫瑰花结形成细胞。用戊二醛聚合对有效的E-RNA提取物进行修饰，导致在测试的两个应答小鼠品系（BDF(1)和ICR瑞士）中，它们诱导对沙门氏菌病抗性的能力显著降低，尽管诱导出了可检测水平的抗体。
