Fujian Provincial Center for Disease Control and Prevention, Fuzhou 350011, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350011, China; Fujian Institute of Preventive Medicine, Fuzhou, 350012, China.
Fujian Provincial Center for Disease Control and Prevention, Fuzhou 350011, China; Fujian Institute of Preventive Medicine, Fuzhou, 350012, China.
J Glob Antimicrob Resist. 2024 Sep;38:106-110. doi: 10.1016/j.jgar.2024.04.003. Epub 2024 May 7.
The objective of this study was to investigate the resistance mechanisms of a multidrug-resistant Salmonella Kentucky ST198 FJ-2064 isolated from a patient in China.
The antimicrobial susceptibility of FJ-2064 was determined by the standard disc dilution and broth microdilution methods. The complete genome of FJ-2064 was sequenced using PacBio and Illumina MiSeq platforms. Polymerase chain reaction (PCR) and S1-PFGE were utilized to confirm the mutation sites and the genomic plasmids, respectively.
Isolate FJ-2064 belongs to sequence type ST198 and harboured no visible large plasmids, but was concurrent resistant to 22 detected antimicrobial agents including cefotaxime, ciprofloxacin, and azithromycin. The complete genome sequence identified 20 acquired antibiotic resistance genes (ARGs) and five chromosomal mutations in the gyrA and parC genes of the quinolone resistance determining regions (QRDRs) in FJ-2064. In addition, PCR sequencing confirmed that most of the ARGs were clustered on one multidrug-resistant region and a variant of SGI1-K. In particular, the bla and bla, qnrS1, mph(A) genes, which confer resistance to cephalosporins, quinolones, and macrolides respectively, were all located on the multidrug-resistant region.
We have demonstrated one multidrug-resistant region and a variant of SGI1-K in a Salmonella Kentucky ST198 that is co-resistant to cefotaxime, ciprofloxacin, and azithromycin.
本研究旨在探讨一株来自中国患者的多重耐药性沙门氏菌 Kentucky ST198 FJ-2064 的耐药机制。
采用标准纸片扩散和肉汤微量稀释法测定 FJ-2064 的药敏性。采用 PacBio 和 Illumina MiSeq 平台对 FJ-2064 的全基因组进行测序。聚合酶链反应（PCR）和 S1-PFGE 分别用于确认突变位点和基因组质粒。
分离株 FJ-2064 属于序列型 ST198，没有明显的大质粒，但同时对 22 种检测到的抗菌药物（包括头孢噻肟、环丙沙星和阿奇霉素）具有耐药性。全基因组序列鉴定出 FJ-2064 中的 20 个获得性抗生素耐药基因（ARGs）和喹诺酮耐药决定区（QRDR）中 gyrA 和 parC 基因的 5 个染色体突变。此外，PCR 测序证实，大多数 ARGs 聚集在一个多药耐药区和 SGI1-K 的一个变体上。特别是，bla 和 bla、qnrS1、mph(A) 基因分别赋予对头孢菌素、喹诺酮类和大环内酯类的耐药性，均位于多药耐药区。
我们已经证明了沙门氏菌 Kentucky ST198 中存在一个多药耐药区和 SGI1-K 的一个变体，该菌对头孢噻肟、环丙沙星和阿奇霉素同时耐药。
