Rajeev Aishwarya, Katukuri Saikumar, Devarashetty Shravya, Dantala Satyanarayana, Billa Aishwarya-Lakshmi
Associate Professor, Department of Oral Pathology and Microbiology, Mahatma Gandhi Dental College & Hospital, Rajasthan, India.
Assistant Professor, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences and Research Centre, Hyderabad, India.
J Clin Exp Dent. 2024 Apr 1;16(4):e391-e398. doi: 10.4317/jced.61057. eCollection 2024 Apr.
An essential molecular occurrence in carcinogenesis that can lead to invasion and migration, predisposing cells to malignant transformation, involves alterations in cell adhesion molecules, such as p120 catenin. The destabilization of E-cadherin, caused by the loss or phosphorylation of p120 catenin (p120), regulates cadherin stability and turnover, impacting cell adhesiveness and migratory capacity. Consequently, p120 is associated with the invasiveness and progression of various human epithelial tumor types, including Oral Squamous Cell Carcinomas (OSCC). The present study aimed to assess and establish a correlation between the expression of p120 antibody in OSCC and Apparently Normal Mucosa Adjacent to OSCC (ANMAOSCC).
The immunoexpression of p120 in 300 selected cases was categorized into two groups: OSCC (n = 150) and ANMAOSCC (n = 150). Two 4µm-thick tissue sections from the selected blocks were prepared. One section was stained with Hematoxylin and Eosin, while the other underwent immunohistochemical (IHC) staining using anti-p120 catenin antibody (clone No. EP66; Catalog No. PR062; PathnSitu, Wayne, PA, USA). The analysis of p120 immunoexpression included parameters such as intensity, percentage, and the location of staining.
In OSCC, over 80% of cases expressed p120, with only 16% exhibiting loss of expression. In ANMAOSCC, all cells maintained p120 expression. In OSCC, p120 was predominantly localized to the membrane and cytoplasm in 76%, while in ANMAOSCC, over 90% showed membrane localization. Regarding positivity, only 19% of OSCC cases reported positivity in >50% of cells, compared to 64.7% in ANMAOSCC. The extent of staining in ANMAOSCC was observed up to the granular layer (45%) and corneal layer (19%).
The atypical staining pattern of p120 may indicate a loss of adhesion and could serve as a marker for identifying the malignant potential of ANMAOSCC and the aggressiveness of OSCC. Oral Squamous Cell Carcinoma, P120 Catenin, Cell Adhesion Molecules, Gene Expression Regulation.
致癌过程中一种关键的分子事件可导致侵袭和迁移，使细胞易于发生恶性转化，这涉及细胞黏附分子的改变，如p120连环蛋白。p120连环蛋白（p120）的缺失或磷酸化导致E-钙黏蛋白不稳定，调节钙黏蛋白的稳定性和周转，影响细胞黏附性和迁移能力。因此，p120与包括口腔鳞状细胞癌（OSCC）在内的多种人类上皮肿瘤类型的侵袭性和进展相关。本研究旨在评估并建立OSCC中p120抗体表达与OSCC相邻的明显正常黏膜（ANMAOSCC）之间的相关性。
将300例选定病例中p120的免疫表达分为两组：OSCC（n = 150）和ANMAOSCC（n = 150）。从选定的组织块中制备两张4μm厚的组织切片。一张切片用苏木精和伊红染色，另一张用抗p120连环蛋白抗体（克隆号EP66；目录号PR062；PathnSitu，美国宾夕法尼亚州韦恩）进行免疫组织化学（IHC）染色。p120免疫表达分析包括强度、百分比和染色位置等参数。
在OSCC中，超过80%的病例表达p120，仅有16%表现为表达缺失。在ANMAOSCC中，所有细胞均维持p120表达。在OSCC中，76%的p120主要定位于细胞膜和细胞质，而在ANMAOSCC中，超过90%表现为膜定位。关于阳性情况，OSCC病例中仅有19%报告>50%的细胞呈阳性，而ANMAOSCC中这一比例为64.7%。在ANMAOSCC中，染色范围可观察到颗粒层（45%）和角质层（19%）。
p120的非典型染色模式可能表明黏附丧失，可作为识别ANMAOSCC恶性潜能和OSCC侵袭性的标志物。口腔鳞状细胞癌、P120连环蛋白、细胞黏附分子、基因表达调控。
