Yang Da Hyun, Lee Nae Yoon
Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Republic of Korea.
Biomed Mater. 2024 May 22;19(4). doi: 10.1088/1748-605X/ad49f7.
In this study, we coated electrospun polycaprolactone (PCL) fibers with polydopamine (PDA) to modify their hydrophobicity and fabricated a matrix for culturing mesenchymal stem cells (MSCs). Additionally, we incorporated Arg-Gly-Asp (RGD) peptides into PDA to enhance MSCs culture performance on PCL fibers. PDA and RGD were successfully coated in one step by immersing the electrospun fibers in a coating solution, without requiring an additional surface activation process. The characteristics of functionalized PCL fibers were analyzed by scanning electron microscopy with energy-dispersive x-ray analysis, Fourier transform infrared spectroscopy, water contact angle measurement, and fluorescence measurements using a carboxylic-modified fluorescent microsphere. MSCs cultured on the modified PCL fibers demonstrated enhanced cell adhesion, proliferation, and osteogenic- and chondrogenic differentiation. This study provides insight into potential applications for scaffold fabrication in MSCs-based tissue engineering, wound dressing, implantation, and a deeper understanding of MSCs behavior.
在本研究中，我们用聚多巴胺（PDA）包覆电纺聚己内酯（PCL）纤维以改变其疏水性，并制备了用于培养间充质干细胞（MSCs）的基质。此外，我们将精氨酸-甘氨酸-天冬氨酸（RGD）肽掺入PDA中，以提高MSCs在PCL纤维上的培养性能。通过将电纺纤维浸入涂层溶液中，一步成功地包覆了PDA和RGD，无需额外的表面活化过程。通过扫描电子显微镜结合能量色散X射线分析、傅里叶变换红外光谱、水接触角测量以及使用羧基修饰的荧光微球进行荧光测量，对功能化PCL纤维的特性进行了分析。在修饰后的PCL纤维上培养的MSCs表现出增强的细胞黏附、增殖以及成骨和成软骨分化能力。本研究为基于MSCs的组织工程中支架制造的潜在应用、伤口敷料、植入提供了见解，并有助于更深入地了解MSCs的行为。
