自发性大鼠大颗粒淋巴细胞白血病细胞系中T细胞受体β链的基因重排及mRNA表达缺失
Lack of gene rearrangement and mRNA expression of the beta chain of the T cell receptor in spontaneous rat large granular lymphocyte leukemia lines.
作者信息
Reynolds C W, Bonyhadi M, Herberman R B, Young H A, Hedrick S M
出版信息
J Exp Med. 1985 May 1;161(5):1249-54. doi: 10.1084/jem.161.5.1249.
Abstract
Using the murine cDNA clone for the beta chain of the T cell antigen receptor, we have examined four highly cytotoxic rat large granular lymphocyte (LGL) leukemia lines for the expression of unique rearrangements and mRNA transcription of the genes coding for the T cell antigen receptor. In contrast to normal rat T cells and nine rat T cell lines, the LGL leukemia lines exhibited no detectable gene rearrangements in the beta chain locus after digestion of LGL DNA by four restriction enzymes. Northern blots containing RNA from these LGL tumor lines demonstrated a low level of aberrant or nonrearranged beta chain transcription (less than 10 copies per cell) but virtually no translatable 1.3 kilobase message. These results demonstrate that LGL leukemia lines which mediate both natural killer (NK) and antibody-dependent cell-mediated cytotoxicity (ADCC) activities do not express the beta chain of the T cell receptor. The nature of the NK cell receptor for antigen remains elusive.
摘要
利用小鼠T细胞抗原受体β链的cDNA克隆，我们检测了四种高细胞毒性大鼠大颗粒淋巴细胞（LGL）白血病细胞系，以了解编码T细胞抗原受体的基因的独特重排和mRNA转录情况。与正常大鼠T细胞和九种大鼠T细胞系不同，在用四种限制性内切酶消化LGL DNA后，LGL白血病细胞系在β链基因座中未表现出可检测到的基因重排。含有这些LGL肿瘤细胞系RNA的Northern印迹显示，β链转录存在低水平的异常或未重排情况（每个细胞少于10个拷贝），但几乎没有可翻译的1.3千碱基信息。这些结果表明，介导自然杀伤（NK）和抗体依赖性细胞介导的细胞毒性（ADCC）活性的LGL白血病细胞系不表达T细胞受体的β链。抗原的NK细胞受体的性质仍然难以捉摸。
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Lack of gene rearrangement and mRNA expression of the beta chain of the T cell receptor in spontaneous rat large granular lymphocyte leukemia lines.自发性大鼠大颗粒淋巴细胞白血病细胞系中T细胞受体β链的基因重排及mRNA表达缺失
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Natural killer (NK) cell activity in the rat. I. Isolation and characterization of the effector cells.大鼠体内的自然杀伤（NK）细胞活性。I. 效应细胞的分离与鉴定。
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Large granular lymphocyte leukemia. A heterogeneous lymphocytic leukemia in F344 rats.大颗粒淋巴细胞白血病。F344大鼠中的一种异质性淋巴细胞白血病。
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