Xin Lei, Chen Yazhen, Rong Wantao, Qin Yingcan, Li Xiaodong, Guan Delong
Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology, Hechi University, Hechi 546300, China.
Guangxi Collaborative Innovation Center of Modern Sericulture and Silk, Hechi University, Hechi 546300, China.
Animals (Basel). 2024 Apr 23;14(9):1261. doi: 10.3390/ani14091261.
The gut microbiome significantly influences the health and productivity of silkworms (), the cornerstone of sericulture. With the increasing use of cost-effective artificial diets in sericulture, it is crucial to understand how these diets impact the silkworm gut microbiomes. Here we employed 16S rRNA sequencing to delineate the impact of three distinct dietary regimens on the silkworm gut microbiomes: exclusive mulberry leaf diet (SY), exclusive artificial feed diet (SL), and a sequential transition from artificial feed to mulberry leaves (ZS). Our results unveiled stark differences in microbial diversity across the groups, with the ZS group displaying an intermediary complexity. LefSe and random forest analyses identified Methylobacteriaceae, , and as significantly enriched in the ZS group, suggesting their potential to facilitate silkworms' adaptation to dietary transitions. Functional profiling revealed differential pathway regulation, indicating a metabolic reconfiguration in response to dietary modulations. Notably, the enrichment of and in both the SL and ZS groups highlights their potential as probiotics in artificial diets. Our findings provide insights into the diet adaptation mechanisms of silkworm gut microbiota, paving the way for harnessing the intestinal bacteria to enhance silkworm health and silk production through targeted microbial interventions in sericulture practices.
肠道微生物群对家蚕（养蚕业的基石）的健康和生产力有显著影响。随着养蚕业中性价比高的人工饲料使用的增加，了解这些饲料如何影响家蚕肠道微生物群至关重要。在此，我们采用16S rRNA测序来描述三种不同饮食方案对家蚕肠道微生物群的影响：纯桑叶饮食（SY）、纯人工饲料饮食（SL）以及从人工饲料到桑叶的顺序转换饮食（ZS）。我们的结果揭示了各组微生物多样性的显著差异，ZS组呈现出中等复杂性。LefSe和随机森林分析确定甲基杆菌科等在ZS组中显著富集，表明它们有助于家蚕适应饮食转换的潜力。功能分析揭示了不同的途径调控，表明响应饮食调节的代谢重新配置。值得注意的是，SL组和ZS组中[具体物质]的富集突出了它们作为人工饲料中益生菌的潜力。我们的研究结果为家蚕肠道微生物群的饮食适应机制提供了见解，为通过养蚕实践中的靶向微生物干预利用肠道细菌来增强家蚕健康和蚕丝生产铺平了道路。
