Wang Xueying, Zhang Bohao, Dong Weitao, Zhao Yu, Zhao Xingxu, Zhang Yong, Zhang Quanwei
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Gansu Key Laboratory of Animal Generational Physiology and Reproductive Regulation, Lanzhou 730070, China.
Animals (Basel). 2024 Apr 24;14(9):1275. doi: 10.3390/ani14091275.
The type II Na/Pi co-transporter (NaPi2b), encoded by the solute carrier (SLC) transporter 34A2 (SLC34A2), is responsible for calcium (Ca) and phosphorus (P) homeostasis. Unbalanced Ca/P metabolism induces mastitis in dairy cows. However, the specific role of SLC34A2 in regulating this imbalance in Holstein cows with clinical mastitis (CM) remains unclear. The aim of this study was to investigate the role of SLC34A2 and identify differentially expressed proteins (DEPs) that interact with SLC34A2 and are associated with Ca/P metabolism in dairy cows with CM. Immunohistochemical and immunofluorescence staining results showed that SLC34A2 was located primarily in the mammary epithelial cells of the mammary alveoli in both the control (healthy cows, Con/C) and CM groups. Compared to the Con/C group, the relative expression of the gene and protein were significantly downregulated in the CM group. We identified 12 important DEPs included in 11 GO terms and two pathways interacting with SLC34A2 using data-independent acquisition proteomics. The PPI (protein-and-protein interaction) network results suggested that these DEPs were associated with ion metabolism and homeostasis, especially SLC34A2. These results demonstrate that SLC34A2 downregulation is negatively correlated with the occurrence and development of CM in Holstein cows, providing a basis for exploring the function and regulatory mechanism of SLC34A2 in Ca/P metabolism and homeostasis in Holstein cows with CM.
由溶质载体（SLC）转运体34A2（SLC34A2）编码的II型钠/磷共转运体（NaPi2b）负责钙（Ca）和磷（P）的体内平衡。钙/磷代谢失衡会诱发奶牛乳腺炎。然而，SLC34A2在调节患有临床乳腺炎（CM）的荷斯坦奶牛这种失衡中的具体作用仍不清楚。本研究的目的是调查SLC34A2的作用，并鉴定与SLC34A2相互作用且与患有CM的奶牛钙/磷代谢相关的差异表达蛋白（DEP）。免疫组织化学和免疫荧光染色结果显示，SLC34A2主要位于对照组（健康奶牛，Con/C）和CM组乳腺腺泡的乳腺上皮细胞中。与Con/C组相比，CM组中该基因和蛋白的相对表达显著下调。我们使用数据非依赖采集蛋白质组学鉴定了11个GO术语和两条与SLC34A2相互作用的途径中包含的12个重要DEP。蛋白质-蛋白质相互作用（PPI）网络结果表明，这些DEP与离子代谢和体内平衡相关，尤其是SLC34A2。这些结果表明，SLC34A2的下调与荷斯坦奶牛CM的发生和发展呈负相关，为探索SLC34A2在患有CM的荷斯坦奶牛钙/磷代谢和体内平衡中的功能及调控机制提供了依据。
