Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 211198, China.
Molecules. 2024 Apr 30;29(9):2067. doi: 10.3390/molecules29092067.
The supramolecular solvent (SUPRAS) has garnered significant attention as an innovative, efficient, and environmentally friendly solvent for the effective extraction and separation of bioactive compounds from natural resources. However, research on the use of a SUPRAS for the extraction of phenolic compounds from plants, which are highly valued in food products due to their exceptional antioxidant properties, remains scarce. The present study developed a green, ultra-sound-assisted SUPRAS method for the simultaneous determination of three phenolic acids in using high-performance liquid chromatography (HPLC). The experimental parameters were meticulously optimized. The efficiency and antioxidant properties of the phenolic compounds obtained using different extraction methods were also compared. Under optimal conditions, the extraction efficiency of the SUPRAS, prepared with octanoic acid reverse micelles dispersed in ethanol-water, significantly exceeded that of conventional organic solvents. Moreover, the SUPRAS method demonstrated greater antioxidant capacity. Confocal laser scanning microscopy (CLSM) images revealed the spherical droplet structure of the SUPRAS, characterized by a well-defined circular fluorescence position, which coincided with the position of the phenolic acids. The phenolic acids were encapsulated within the SUPRAS droplets, indicating their efficient extraction capacity. Furthermore, molecular dynamics simulations combined with CLSM supported the proposed method's mechanism and theoretically demonstrated the superior extraction performance of the SUPRAS. In contrast to conventional methods, the higher extraction efficiency of the SUPRAS can be attributed to the larger solvent contact surface area, the formation of more types of hydrogen bonds between the extractants and the supramolecular solvents, and stronger, more stable interaction forces. The results of the theoretical studies corroborate the experimental outcomes.
超分子溶剂 (SUPRAS) 作为一种创新、高效、环保的溶剂，在有效提取和分离天然资源中的生物活性化合物方面引起了广泛关注。然而，关于 SUPRAS 用于从植物中提取酚类化合物的研究却很少，而这些化合物由于其出色的抗氧化性能，在食品产品中具有很高的价值。本研究开发了一种绿色、超声辅助的 SUPRAS 方法，用于使用高效液相色谱法 (HPLC) 同时测定 中的三种酚酸。对实验参数进行了精心优化。还比较了使用不同提取方法获得的酚类化合物的效率和抗氧化性能。在最佳条件下，用辛酸酸反向胶束分散在乙醇-水中制备的 SUPRAS 的提取效率明显高于传统有机溶剂。此外，SUPRAS 方法表现出更大的抗氧化能力。共焦激光扫描显微镜 (CLSM) 图像显示了 SUPRAS 的球形液滴结构，具有定义明确的圆形荧光位置，与酚酸的位置一致。酚酸被包裹在 SUPRAS 液滴内，表明其具有高效的提取能力。此外，分子动力学模拟结合 CLSM 支持了所提出方法的机制，并从理论上证明了 SUPRAS 的优越提取性能。与传统方法相比，SUPRAS 更高的提取效率可归因于更大的溶剂接触表面积、萃取剂与超分子溶剂之间形成更多类型的氢键以及更强、更稳定的相互作用力。理论研究的结果与实验结果相符。
