Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Liege, 5030 Gembloux, Belgium.
Molecules. 2024 May 1;29(9):2096. doi: 10.3390/molecules29092096.
We aimed to obtain the optimal formula for human milk fat substitute (HMFS) through a combination of software and an evaluation model and further verify its practicability through an animal experiment. The results showed that a total of 33 fatty acid (FA) and 63 triglyceride (TAG) molecular species were detected in vegetable oils. Palmitic acid, oleic acid, linoleic acid, 18:1/16:0/18:1, 18:2/16:0/18:2, 18:1/18:1/18:1 and 18:1/18:2/18:1, were the main molecular species among the FAs and TAGs in the vegetable oils. Based on the HMFS evaluation model, the optimal mixed vegetable oil formula was blended with 21.3% palm oil, 2.8% linseed oil, 2.6% soybean oil, 29.9% rapeseed oil and 43.4% maize oil, with the highest score of 83.146. Moreover, there was no difference in the weight, blood routine indices or calcium and magnesium concentrations in the feces of the mice between the homemade mixed vegetable oil (HMVO) group and the commercial mixed vegetable oil (CMVO) group, while nervonic acid (C24:1) and octanoic acid (C8:0) were absorbed easily in the HMVO group. Therefore, these results demonstrate that the mixing of the different vegetable oils was feasible via a combination of computer software and an evaluation model and provided a new way to produce HMFS.
本研究旨在通过软件与评价模型相结合，获得人乳脂肪替代品（HMFS）的最佳配方，并通过动物实验进一步验证其可行性。结果表明，植物油中共检测到 33 种脂肪酸（FA）和 63 种三酰基甘油（TAG）分子种类。棕榈酸、油酸、亚油酸、18:1/16:0/18:1、18:2/16:0/18:2、18:1/18:1/18:1 和 18:1/18:2/18:1 是植物油中 FA 和 TAG 的主要分子种类。基于 HMFS 评价模型，将 21.3%的棕榈油、2.8%的亚麻籽油、2.6%的大豆油、29.9%的菜籽油和 43.4%的玉米油混合，得到最佳混合植物油配方，其评分最高，为 83.146。此外，与商业混合植物油（CMVO）组相比，自制混合植物油（HMVO）组的小鼠体重、血常规指标或粪便中钙镁浓度均无差异，而神经酸（C24:1）和辛酸（C8:0）在 HMVO 组中易于吸收。因此，这些结果表明，通过计算机软件与评价模型的结合，不同植物油的混合是可行的，为生产 HMFS 提供了一种新方法。
