Laboratory of Physiology, Department for Sustainable Development and Ecological Transition, 13100 Vercelli, Italy.
Noivita Srls, Spin Off of the University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy.
Int J Mol Sci. 2024 Apr 27;25(9):4774. doi: 10.3390/ijms25094774.
The gut-brain axis is a bidirectional relationship between the microbiota and the brain; genes related to the brain and gut synaptic formation are similar. Research on the causal effects of gut microbiota on human behavior, brain development, and function, as well as the underlying molecular processes, has emerged in recent decades. Probiotics have been shown in several trials to help reduce anxiety and depressive symptoms. Because of this, probiotic combinations have been tested in in vitro models to see whether they might modulate the gut and alleviate depression and anxiety. Therefore, we sought to determine whether a novel formulation might affect the pathways controlling anxiety and depression states and alter gut barrier activities in a 3D model without having harmful side effects. Our findings indicate that novaBBF7 10 mg/mL, novaBLG2 5 mg/mL, and TJB8 10 mg/mL may influence the intestinal barrier and enhance the synthesis of short-chain fatty acids. Additionally, the probiotics studied did not cause neuronal damage and, in combination, exert a protective effect against the condition of anxiety and depression triggered by L-Glutamate. All these findings show that probiotics can affect gut function to alter the pathways underlying anxiety and depression.
肠脑轴是微生物群和大脑之间的双向关系；与大脑和肠道突触形成相关的基因是相似的。近几十年来，人们对肠道微生物群对人类行为、大脑发育和功能的因果影响以及潜在的分子过程进行了研究。多项试验表明，益生菌有助于减轻焦虑和抑郁症状。因此，人们已经在体外模型中测试了益生菌组合，以观察它们是否可以调节肠道，缓解抑郁和焦虑。因此，我们试图确定一种新的配方是否可能影响控制焦虑和抑郁状态的途径，并在没有有害副作用的情况下改变 3D 模型中的肠道屏障活动。我们的研究结果表明，novaBBF7 10mg/ml、novaBLG2 5mg/ml 和 TJB8 10mg/ml 可能影响肠道屏障并增强短链脂肪酸的合成。此外，所研究的益生菌不会引起神经元损伤，并且联合使用时对由 L-谷氨酸引发的焦虑和抑郁状态具有保护作用。所有这些发现表明，益生菌可以影响肠道功能，从而改变焦虑和抑郁的潜在途径。
