Laboratory of Physiology, Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy.
Noivita Srls, Spin Off, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy.
Int J Mol Sci. 2023 Jul 31;24(15):12281. doi: 10.3390/ijms241512281.
Despite the identification of several innovative targets for avoiding cognitive decline, there has yet to be a widely accepted approach that deals with minimising the deterioration of cognitive function. In this light, recent studies suggest that regulating the gut-brain axis with probiotics is a potential therapeutic strategy to support brain health. For this reason, in vitro models were used to examine the efficacy of different probiotic combinations to enhance intestinal homeostasis and positively affect the brain. Therefore, the new formulation has been evaluated for its capacity to modify intestinal barrier functions in a 3D in vitro model without any adverse effects and directly impact the mechanisms underlying cognitive function in a gut-brain axis model. According to our findings, novaBBF7 10 mg/mL, novaBLG2 5 mg/mL and TJB8 10 mg/mL may successfully modify the intestinal barrier and improve SCFA production. Successively, the probiotics studied caused no harm at the neuronal level, as demonstrated by iNOS, mitochondrial potential, and cell viability tests, confirming their safety features and enhancing antioxidant mechanisms and antineuroinflammation activity. Additionally, the damage caused by oxidative stress was also healed, and critical pathways that result in cognitive impairment were changed by synergetic action, supporting the hypothesis that brain ageing and neurodegeneration are slowed down. All these findings demonstrate the ability of probiotics to affect cognitive processes and their ability to sustain the mechanisms underlying cognitive function by acting on intestinal function.
尽管已经确定了几个避免认知能力下降的创新靶点，但目前还没有一种被广泛接受的方法来尽量减少认知功能的恶化。有鉴于此，最近的研究表明，通过益生菌来调节肠脑轴可能是支持大脑健康的一种潜在治疗策略。出于这个原因，使用体外模型来研究不同益生菌组合增强肠道内稳态并对大脑产生积极影响的功效。因此，评估了新配方在没有任何不良反应的情况下在 3D 体外模型中改变肠道屏障功能的能力，并直接影响肠脑轴模型中认知功能的潜在机制。根据我们的发现，novaBBF7 10 mg/mL、novaBLG2 5 mg/mL 和 TJB8 10 mg/mL 可能成功地改变肠道屏障并提高 SCFA 的产生。随后，通过 iNOS、线粒体电位和细胞活力测试证明，研究中的益生菌在神经元水平上没有造成任何伤害，证实了它们的安全性特征，并增强了抗氧化机制和抗神经炎症活性。此外，还修复了氧化应激引起的损伤，通过协同作用改变了导致认知障碍的关键途径，支持了大脑衰老和神经退行性变减缓的假说。所有这些发现都表明益生菌能够影响认知过程，并通过作用于肠道功能来维持认知功能的潜在机制。
